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Syllabus 2024-25 - 76712004 - Advanced Techniques for Cell Biology (Técnicas avanzadas en biología celular)
- Level 1: Tutorial support sessions, materials and exams in this language
- Level 2: Tutorial support sessions, materials, exams and seminars in this language
- Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
| DEGREE: | Master's Degree in Biotechnology and Biomedicine (76712004) |
| FACULTY: | Centro de Estudios de Postgrado |
| DEGREE: | MDs in Biotechnology and Biomedicine + Secondary Teacher Training (77912003) |
| FACULTY: | Centro de Estudios de Postgrado |
| ACADEMIC YEAR: | 2024-25 |
| COURSE: | Advanced Techniques for Cell Biology |
| NAME: Advanced Techniques for Cell Biology | |||||
| CODE: 76712004 (*) | ACADEMIC YEAR: 2024-25 | ||||
| LANGUAGE: English | LEVEL: 1 | ||||
| ECTS CREDITS: 6.0 | YEAR: 1 | SEMESTER: PC | |||
| NAME: TORRES LÓPEZ, MARÍA ISABEL | ||
| DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL | ||
| FIELD OF STUDY: 050 - BIOLOGÍA CELULAR | ||
| OFFICE NO.: B3 - B3-361 | E-MAIL: mitorres@ujaen.es | P: 212762 |
| WEBSITE: - | ||
| ORCID: https://orcid.org/0000-0002-9309-3837 | ||
| LANGUAGE: - | LEVEL: 1 | |
Introduce the student to the techniques related to animal cell cultures, as well as their applications in the field of biomedical and biotechnological research. Knowledge of the importance of using cultured cells in research. Clinical and industrial utility.
THEMATIC BLOCK I
Know the fundamentals and management of the different types of microscopes and their application in the different fields of research, as well as the most common methods of capturing and processing the images obtained.
The biological light microscope. Optical and mechanical components.
Other types of optical microscopes, elements that integrate them, handling and applications of the same
Electron microscopes: basics of their operation and components.
Sample preparation methods for different types of electron microscopes.
Specialized techniques in detection and analysis applied to microscopy.
Image capture systems in optical and electron microscopy
Treatment and management of digital images.. Fundamentals of image analysis and management of computer applications for this purpose
Photographic reconstruction techniques in optical microscopy, both in 2D and 3D.
THEMATIC BLOCK II
Introduction. Biology of cells in culture. Minimum equipment and practical considerations. Cell cultures as an alternative method to animal experimentation.
Cell culture types. Culture media. Substrates: role of the extracellular matrix. Role of serum in cell cultures.
Cell lines. Growth and maintenance of cells in culture. Subculture of cell lines. Freezing and thawing.
Cellular sync. Stimulation by growth factors of the quiescence-proliferation step. Study of the cell cycle.
Cell differentiation
cell transfection.
Use of fluorescence microscopy and confocal microscopy
The contents of this subject connect with SDG-4: Guarantee inclusive, equitable and quality education and promote lifelong learning opportunities for all. SDG-5: Achieve gender equality and empower all women and girls. SDG-3: Guarantee a healthy life and promote well-being for all at all ages. SDG-8: Promote inclusive and sustainable economic growth, employment and decent work for all
Expository classes:
Introductory activities.
Master session.
Conferences.
Classes in practice groups:
Practices Laboratory.
Seminars.
Discussions.
Resolution of exercises.
Presentations/exhibitions.
Group/individual tutorials: Supervision of directed works. Seminars. Discussions
Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support
The qualification system will be governed by the provisions of RD 1125/2003 of September 5, which establishes the European credit system and the qualification system in official university degrees.
- Culture of animal cells : a manual of basic technique and specialized applications. Edition: 6th ed. Author: Freshney, R. Ian. Publisher: Hoboken, NJ : Wiley-Blackwell, cop. 2010 (Library)
- Basic cell culture : a practical approach. Edition: 2nd. ed., reimpr. Author: -. Publisher: Oxford ; New York : Oxford University, 2006 (Library)
- Animal cell culture methods. Edition: -. Author: -. Publisher: San Diego [etc.]: Academic Press, cop. 1998 (Library)
- Optical Imaging and Microscopy [Recurso electrónico] : Techniques and Advanced Systems. Edition: 2. Author: Török, Peter. Publisher: Berlin, Heidelberg : Springer-Verlag, 2007 (Library)
- Técnicas en histología y biología celular. Edition: 2ª ed. Author: Montuenga Badía, Luis. Publisher: Elsevier-Masson, 2014 (Library)
- Electron microscopy: principles and techniques for biologists. Edition: 2nd ed. Author: Bozzola, John J.. Publisher: Boston: Jones and Bartlett, cop. 1999 (Library)
- Microscopy, immunohistochemistry, and antigen retrieval methods: for light and electron microscopy. Edition: -. Author: Hayat, M. A.. Publisher: New York [etc.]: Kluwer Academic Plenum Publishers, cop. 2002 (Library)
- Culture of animal cells : a manual of basic technique and specialized applications. Edition: 6th ed. Author: Freshney, R. Ian. Publisher: Hoboken, NJ : Wiley-Blackwell, cop. 2010 (Library)
- Basic cell culture : a practical approach. Edition: 2nd. ed., reimpr. Author: -. Publisher: Oxford ; New York : Oxford University, 2006 (Library)
- Animal cell culture methods. Edition: -. Author: -. Publisher: San Diego [etc.]: Academic Press, cop. 1998 (Library)
- Optical Imaging and Microscopy [Recurso electrónico] : Techniques and Advanced Systems. Edition: 2. Author: Török, Peter. Publisher: Berlin, Heidelberg : Springer-Verlag, 2007 (Library)
- Técnicas en histología y biología celular. Edition: 2ª ed. Author: Montuenga Badía, Luis. Publisher: Elsevier-Masson, 2014 (Library)
- Electron microscopy: principles and techniques for biologists. Edition: 2nd ed. Author: Bozzola, John J.. Publisher: Boston: Jones and Bartlett, cop. 1999 (Library)
- Microscopy, immunohistochemistry, and antigen retrieval methods: for light and electron microscopy. Edition: -. Author: Hayat, M. A.. Publisher: New York [etc.]: Kluwer Academic Plenum Publishers, cop. 2002 (Library)
- Culture of animal cells : a manual of basic technique and specialized applications. Edition: 6th ed. Author: Freshney, R. Ian. Publisher: Hoboken, NJ : Wiley-Blackwell, cop. 2010 (Library)
- Basic cell culture : a practical approach. Edition: 2nd. ed., reimpr. Author: -. Publisher: Oxford ; New York : Oxford University, 2006 (Library)
- Animal cell culture methods. Edition: -. Author: -. Publisher: San Diego [etc.]: Academic Press, cop. 1998 (Library)
- Optical Imaging and Microscopy [Recurso electrónico] : Techniques and Advanced Systems. Edition: 2. Author: Török, Peter. Publisher: Berlin, Heidelberg : Springer-Verlag, 2007 (Library)
- Técnicas en histología y biología celular. Edition: 2ª ed. Author: Montuenga Badía, Luis. Publisher: Elsevier-Masson, 2014 (Library)
- Electron microscopy: principles and techniques for biologists. Edition: 2nd ed. Author: Bozzola, John J.. Publisher: Boston: Jones and Bartlett, cop. 1999 (Library)
- Microscopy, immunohistochemistry, and antigen retrieval methods: for light and electron microscopy. Edition: -. Author: Hayat, M. A.. Publisher: New York [etc.]: Kluwer Academic Plenum Publishers, cop. 2002 (Library)
- Tissue Engineering III: Cell - Surface Interactions for Tissue Culture [Recurso electrónico]. Edition: -. Author: Kasper, Cornelia. Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg, 2012 (Library)
- http://www.nobelprize.org/educational/physics/microscopes/tem/tem.html. Edition: -. Author: - (Library)
- ◾ http://micro.magnet.fsu.edu/primer/virtual/confocal/index.html.. Edition: -. Author: - (Library)
- ◾ http://www.microscopyu.com/tutorials/java/phasecontrast/opticaltrain/index.html. Edition: -. Author: - (Library)
- http://www.olympusconfocal.com/java/confocalvswidefield/index.html. Edition: -. Author: - (Library)
- http://rsbweb.nih.gov/ij/. Edition: -. Author: - (Library)
- http://imagej.nih.gov/ij/. Edition: -. Author: - (Library)
- Cell culture technology for pharmaceutical and cell-based therapies. Edition: -. Author: -. Publisher: Boca Ratón: Taylor & Francis, 2006 (Library)
- Tissue Engineering III: Cell - Surface Interactions for Tissue Culture [Recurso electrónico]. Edition: -. Author: Kasper, Cornelia. Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg, 2012 (Library)
- http://www.nobelprize.org/educational/physics/microscopes/tem/tem.html. Edition: -. Author: - (Library)
- ◾ http://micro.magnet.fsu.edu/primer/virtual/confocal/index.html.. Edition: -. Author: - (Library)
- ◾ http://www.microscopyu.com/tutorials/java/phasecontrast/opticaltrain/index.html. Edition: -. Author: - (Library)
- http://www.olympusconfocal.com/java/confocalvswidefield/index.html. Edition: -. Author: - (Library)
- http://rsbweb.nih.gov/ij/. Edition: -. Author: - (Library)
- http://imagej.nih.gov/ij/. Edition: -. Author: - (Library)
- Cell culture technology for pharmaceutical and cell-based therapies. Edition: -. Author: -. Publisher: Boca Ratón: Taylor & Francis, 2006 (Library)
- Tissue Engineering III: Cell - Surface Interactions for Tissue Culture [Recurso electrónico]. Edition: -. Author: Kasper, Cornelia. Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg, 2012 (Library)
- http://www.nobelprize.org/educational/physics/microscopes/tem/tem.html. Edition: -. Author: - (Library)
- ◾ http://micro.magnet.fsu.edu/primer/virtual/confocal/index.html.. Edition: -. Author: - (Library)
- ◾ http://www.microscopyu.com/tutorials/java/phasecontrast/opticaltrain/index.html. Edition: -. Author: - (Library)
- http://www.olympusconfocal.com/java/confocalvswidefield/index.html. Edition: -. Author: - (Library)
- http://rsbweb.nih.gov/ij/. Edition: -. Author: - (Library)
- http://imagej.nih.gov/ij/. Edition: -. Author: - (Library)
- Cell culture technology for pharmaceutical and cell-based therapies. Edition: -. Author: -. Publisher: Boca Ratón: Taylor & Francis, 2006 (Library)
| Salud y bienestar |
| Educación de calidad |
| Igualdad de género |
| Trabajo decente y crecimiento económico |
| Producción y consumo responsables |
Goal 3: Good Health and Well-being. Ensure healthy lives and promote well-being for all at all ages
3.b Support the research and development of vaccines and medicines for communicable and non-communicable diseases that primarily affect developing countries, and provide access to affordable essential medicines and vaccines in accordance with the Doha Declaration on the TRIPS Agreement and Public Health, which affirms the right of developing countries to use the full provisions of the TRIPS Agreement regarding flexibility to protect public health, and, in particular, to provide access to medicines for all.
3.c Substantially increase health financing and the recruitment, development, training, and retention of the health workforce in developing countries, especially in the least developed countries and small island developing States.
3.d Strengthen the capacity of all countries, particularly developing countries, for early warning, risk reduction, and management of national and global health risks.
Goal 4: Quality Education. Ensure inclusive and equitable quality education and promote lifelong learning opportunities for all
4.4 By 2030, substantially increase the number of youth and adults who have relevant skills, including technical and vocational skills, for employment, decent jobs, and entrepreneurship.
4.5 By 2030, eliminate gender disparities in education and ensure equal access to all levels of education and vocational training for the vulnerable, including persons with disabilities, indigenous peoples, and children in vulnerable situations.
4.7 By 2030, ensure that all learners acquire the knowledge and skills needed to promote sustainable development, including, among others, through education for sustainable development and sustainable lifestyles, human rights, gender equality, promotion of a culture of peace and non-violence, global citizenship, and appreciation of cultural diversity and of culture’s contribution to sustainable development.
4.a Build and upgrade education facilities that are child, disability, and gender-sensitive, and provide safe, non-violent, inclusive, and effective learning environments for all.
Goal 5: Gender Equality. Achieve gender equality and empower all women and girls
5.1 End all forms of discrimination against all women and girls everywhere.
5.2 Eliminate all forms of violence against all women and girls in public and private spheres, including trafficking and sexual and other types of exploitation.
5.5 Ensure women’s full and effective participation and equal opportunities for leadership at all levels of decision-making in political, economic, and public life.
5.a Undertake reforms to give women equal rights to economic resources, as well as access to ownership and control over land and other forms of property, financial services, inheritance, and natural resources, in accordance with national laws.
5.b Enhance the use of enabling technology, in particular information and communications technology, to promote the empowerment of women.
5.c Adopt and strengthen sound policies and enforceable legislation for the promotion of gender equality and the empowerment of all women and girls at all levels.
Goal 8: Decent Work and Economic Growth. Promote sustained, inclusive, and sustainable economic growth, full and productive employment, and decent work for all
8.4 Improve progressively, by 2030, global resource efficiency in consumption and production, and endeavor to decouple economic growth from environmental degradation, in accordance with the 10-Year Framework of Programmes on Sustainable Consumption and Production, with developed countries taking the lead.
8.5 By 2030, achieve full and productive employment and decent work for all women and men, including for young people and persons with disabilities, and equal pay for work of equal value.
8.6 By 2020, substantially reduce the proportion of youth not in employment, education, or training.
8.7 Take immediate and effective measures to eradicate forced labor, end modern slavery and human trafficking, and secure the prohibition and elimination of the worst forms of child labor, including recruitment and use of child soldiers, and by 2025 end child labor in all its forms.
8.8 Protect labor rights and promote safe and secure working environments for all workers, including migrant workers, particularly women migrants, and those in precarious employment.
Goal 16: Peace, Justice, and Strong Institutions. Promote peaceful and inclusive societies for sustainable development, provide access to justice for all, and build effective, accountable, and inclusive institutions at all levels
16.1 Significantly reduce all forms of violence and related death rates everywhere.
16.2 End abuse, exploitation, trafficking, and all forms of violence against and torture of children.
16.3 Promote the rule of law at the national and international levels and ensure equal access to justice for all.
16.10 Ensure public access to information and protect fundamental freedoms, in accordance with national legislation and international agreements.
Teaching methodology
The theory classes in a large group will be carried out 100% in person if there are classrooms with enough space to maintain the safety distance. If this is not the case, they will be carried out semi-attendance at 50% and alternate. Half of the large group will attend a given class in person and the rest will attend via online videoconference, and in the next class, attendance is alternated.
The practices will be carried out in the laboratory in a small group with 100% presential attendance, if space is available that allows the safety distance to be maintained. If this is not the case, they will be carried out in a semi-presential manner at 50% and alternately, transmitting the session to non-attendees by videoconference. After each session and outside the laboratory, students must answer a questionnaire related to the corresponding practice.
The tutorials, individual or collective, will be carried out synchronously by videoconference or asynchronously through the Virtual Teaching forum.
Evaluation systems
The criteria and evaluation systems of presential scenario are maintained, both in the ordinary and extraordinary convocatory.
Means
Virtual teaching platform
Google-meet
Teaching methodology
The large group theory classes will be held via synchronous online videoconference.
The laboratory practice sessions will be replaced by video demonstrations, directed to the small group by videoconference and tutored by the teaching staff. The videos will reflect the development of the experimental protocols proposed in the subject. The students will receive information and feasible results with the experimentation carried out in the demonstrations, which they will have to manage to respond to a series of exercises that must be delivered to the teaching staff for evaluation. The means for the delivery of exercises will be the Virtual Teaching platform.
The tutorials, individual or collective, will be carried out synchronously by videoconference or asynchronously through the Virtual Teaching forum.
Evaluation systems
The criteria and evaluation systems of the presential scenario are maintained, both in the ordinary and extraordinary convocatory.
Means
Virtual teaching platform
Google-meet
Online videos of theory master classes.
Institution in charge of data processing: Universidad de Jaén, Campus Las Lagunillas, s/n, 23071 Jaén
Data Protection Delegate: dpo@ujaen.es
Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Jaén to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.
Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.
Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Jaén has signed the corresponding data access contracts.
Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.
Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es
Person in charge: Universidad de Jaén, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es
Data protection delegate (DPO): TELEFÓNICA, S.A.U. ; Email: dpo@ujaen.es
Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching
Period for record storage: Images will be kept during legal term according to regulations in force
Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents
Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality
Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es