Universidad de Jaén

PROGRAM OF THEORY

1: Introduction to Biochemistry. Water. pH. Buffers.

2: Carbohydrates

3: Lipids

4: Nucleotides. Nucleic acids: DNA and RNA. Structure of nucleic acids.

5: Technical handling of nucleic acids. Purification. Hybridization. The polymerase chain reaction. Recombinant DNA technology. Genomics.

6: Amino acids. The peptide bond. Protein structures.

7: Structure-function relationships of proteins

8: Proteomic

9: Enzymes

10: Thermodynamics of biochemical processes. Gibbs free energy. Energy of biological redox reactions.

11: Metabolism: Metabolic pathways. Catabolism and anabolism. General mechanisms of metabolic regulation.

12: The Krebs cycle. Anaplerotic reactions. Ketones.

13: Oxidative phosphorylation. Photophosphorylation.

14: Metabolism of carbohydrates: Glycolysis. Fermentations. Glycogen synthesis and degradation. Gluconeogenesis. Pentose phosphate pathway. The Calvin-Beson cycle.

15: Lipid Metabolism: Degradation and synthesis of fatty acid. Synthesis of triacylglycerols and glycerophospholipids. Cholesterol synthesis. Lipid mobilization in human. Lipoproteins.

16: Metabolism of nitrogenous compounds: Degradation of proteins and amino acids. Deamination and transamination. The urea cycle. Nucleotide synthesis and degradation.

17: Metabolic profile of the main human organs. Metabolomic.

18: DNA replication.

19: Transcription: biological synthesis of RNA

20: Translation: biological synthesis of proteins.

PROGRAM OF LABORATORY

Session 1: Polymerase chain reaction and DNA electrophoresis in agarose gel.

Session 2: Purification of a protein by affinity chromatography.

Session 3: Protein electrophoresis (SDS-PAGE)

Session 4: Kinetic characterization of an enzyme activity.

Session 5: Western-blot

Session 6: Analysis of glycogen.

Session 7: Analysis of triacylglycerols and cholesterol in blood samples. Analysis of lipid peroxidation derivatives in foods.

Theory classes.

Practical laboratory sessions in small groups where the student will apply different techniques and experimental protocols related to the theoretical contents. The student will have to collect and discuss the obtained results after each practical session.

Practical activities and problem resolution in small groups.

Individual and collective tutorial sessions, where the student will be able to ask and solve questions guided by the professor.

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

Theoretical knowledge will be evaluated from the written answer to the exam questions. To pass Theory, a grade of 5 out of 10 must be obtained. If the Theory is not approved, the subject is not approved.

The practices will be evaluated based on the attitude in the laboratory and the answer to questions and exercises related to each practice. Only the work of those attending the practices will be evaluated. Failure to attend a practical session, if not justified, implies obtaining a zero in the grade corresponding to the activity, with the corresponding decrease in the general grade. If you do not attend at least five laboratory practice sessions, you will not have a grade in the Practices aspect. Also, there will be a written exam related to practical activities.

Problem-based learning (PBL) will be evaluated based on the results of the tasks proposed in each session. Only the work of those attending the PBL sessions will be evaluated. Failure to attend a PBL session, if not justified, implies obtaining a zero in the grade corresponding to the activity, with the corresponding decrease in the general grade. If you do not attend at least five PBL sessions, you will not be graded in this aspect. There will also be a written exam related to the PBL activities carried out.

In the MIXED SCENARIO, both teaching and evaluation will take place in person to the extent that conditions allow.

Methodology:

Theory classes will be held in person in the classroom.

The practices will be carried out in the laboratory in a small group. Otherwise, half of the small group will carry out a face-to-face practical session in the laboratory and the other half will attend videos and online tutorials that show the fundamentals and procedures of an internship in the schedule established by the Faculty. In the next practical session, the assistance procedure is alternated. Students must answer questions and exercises related to the corresponding practical sessions and deliver them to the teaching staff through the Virtual Teaching platform.

The classes of Based on Problems Learning  (BPL) will be carried out  face-to-face, in small group in classrooms with sufficient capacity to maintain the safety distance.

The tutoring sessions, individual or collective, will be carried out synchronously through videoconference or asynchronous through the Virtual Teaching forum.

Evaluation system

The evaluation criteria and procedures are maintained for the theory, practical and BPL activities that exist in the face-to-face setting. Theory must be passed in order to pass the subject.

Resources:

Virtual Teaching platform (PLATEA)

Google Meet

Online videos of theory lectures.

Game based learning: Kahoot, Hot potatoes, Biomodel, etc.

In the NON-PRESENTIAL SCENARIO the activities will be carried out online.

Methodology

Theoretical classes to the whole group by videoconference in the schedule established by the Faculty.

The laboratory sessions will be replaced by video demonstrations or videoconference. The students will receive information and feasible results with the experimentation carried out in the demonstrations, which they must manage to respond to a series of exercises that must be delivered to the teachers for evaluation through the Virtual Teaching platform.

The directed activities (BPL) will be carried out in small groups in a tutored way by videoconference. It will be required the answering and delivery of a questionnaire.

The tutored sessions, individual or collective, will be carried out synchronously through videoconference or could be asynchronous through the Virtual Teaching forum.

Evaluation system

The theoretical exams will be carried out using online forms of multiple choice and short answer. Questions must be answered in a limited time. The teachers will monitor the completion of the exam for each student by videoconference.

The works and exercises of the virtual practical sessions and PBL are evaluated, and must be delivered within a period stipulated by the teaching staff.

Theory must be passed in order to pass the subject.

Resources:

Virtual Teaching platform (PLATEA)

Google Meet

Online videos of theory lectures.

Game based learning: Kahoot, Hot potatoes, Biomodel, etc.

Institution in charge of data processing: Universidad de Jaén, Campus Las Lagunillas, s/n, 23071 Jaén

Data Protection Delegate: dpo@ujaen.es

Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Jaén to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.

Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.

Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Jaén has signed the corresponding data access contracts.

Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.

Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es

Person in charge: Universidad de Jaén, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es

Data protection delegate (DPO): TELEFÓNICA, S.A.U. ; Email: dpo@ujaen.es

Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching

Period for record storage: Images will be kept during legal term according to regulations in force

Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents

Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality

Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es