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Syllabus 2022-23 - 76712004 - Advanced Techniques for Cell Biology (Técnicas avanzadas en biología celular)

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  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Máster Univ. en Biotecnología y biomedicina (76712004)
FACULTY: Centro de Estudios de Postgrado
DEGREE: Doble Máster en Biotecnología y Biomedicina y MFPES (Profesorado) (77912003)
FACULTY: Centro de Estudios de Postgrado
ACADEMIC YEAR: 2022-23
COURSE: Advanced Techniques for Cell Biology
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Advanced Techniques for Cell Biology
CODE: 76712004 (*) ACADEMIC YEAR: 2022-23
LANGUAGE: English LEVEL: 2
ECTS CREDITS: 6.0 YEAR: - SEMESTER: PC
2. LECTURER BASIC INFORMATION
NAME: TORRES LÓPEZ, MARÍA ISABEL
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 050 - BIOLOGÍA CELULAR
OFFICE NO.: B3 - B3-361 E-MAIL: mitorres@ujaen.es P: 212762
WEBSITE: -
ORCID: https://orcid.org/0000-0002-9309-3837
LANGUAGE: - LEVEL: 2
3. CONTENT DESCRIPTION

Introduce the student to the techniques related to animal cell cultures, as well as their applications in the field of biomedical and biotechnological research. Knowledge of the importance of using cultured cells in research. Clinical and industrial utility.

 

THEMATIC BLOCK I

 

Know the fundamentals and management of the different types of microscopes and their application in the different fields of research, as well as the most common methods of capturing and processing the images obtained.

 

The biological light microscope. Optical and mechanical components.

 

Other types of optical microscopes, elements that integrate them, handling and applications of the same

 

Electron microscopes: basics of their operation and components.

 

Sample preparation methods for different types of electron microscopes.

 

Specialized techniques in detection and analysis applied to microscopy.

 

Image capture systems in optical and electron microscopy

 

Treatment and management of digital images.. Fundamentals of image analysis and management of computer applications for this purpose

 

Photographic reconstruction techniques in optical microscopy, both in 2D and 3D.

 

  THEMATIC BLOCK II

 

Introduction. Biology of cells in culture. Minimum equipment and practical considerations. Cell cultures as an alternative method to animal experimentation.

 

Cell culture types. Culture media. Substrates: role of the extracellular matrix. Role of serum in cell cultures.

 

Cell lines. Growth and maintenance of cells in culture. Subculture of cell lines. Freezing and thawing.

 

Cellular sync. Stimulation by growth factors of the quiescence-proliferation step. Study of the cell cycle.

 

Cell differentiation

 

cell transfection.

 

Use of fluorescence microscopy and confocal microscopy

 

The contents of this subject connect with SDG-4: Guarantee inclusive, equitable and quality education and promote lifelong learning opportunities for all. SDG-5: Achieve gender equality and empower all women and girls. SDG-3: Guarantee a healthy life and promote well-being for all at all ages. SDG-8: Promote inclusive and sustainable economic growth, employment and decent work for all

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

Expository classes:

 Introductory activities.

Master session.

Conferences.

 

Classes in practice groups:

 Practices Laboratory.

Seminars.

Discussions.

Resolution of exercises.

Presentations/exhibitions.

Group/individual tutorials: Supervision of directed works. Seminars. Discussions

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

The qualification system will be governed by the provisions of RD 1125/2003 of September 5, which establishes the European credit system and the qualification system in official university degrees.

6. BOOKLIST
MAIN BOOKLIST:
  • Optical Imaging and Microscopy [Recurso electrónico] : Techniques and Advanced Systems. Edition: 2. Author: Török, Peter. Publisher: Berlin, Heidelberg : Springer-Verlag, 2007  (Library)
  • Técnicas en histología y biología celular. Edition: 2ª ed. Author: Montuenga Badía, Luis. Publisher: Elsevier-Masson, 2014  (Library)
  • Electron microscopy: principles and techniques for biologists. Edition: 2nd ed. Author: Bozzola, John J.. Publisher: Boston: Jones and Bartlett, cop. 1999  (Library)
  • Microscopy, immunohistochemistry, and antigen retrieval methods: for light and electron microscopy. Edition: -. Author: Hayat, M. A.. Publisher: New York [etc.]: Kluwer Academic Plenum Publishers, cop. 2002  (Library)
  • Culture of animal cells : a manual of basic technique and specialized applications. Edition: 6th ed. Author: Freshney, R. Ian. Publisher: Hoboken, NJ : Wiley-Blackwell, cop. 2010  (Library)
  • Basic cell culture : a practical approach. Edition: 2nd. ed., reimpr. Author: -. Publisher: Oxford ; New York : Oxford University, 2006  (Library)
  • Animal cell culture methods. Edition: -. Author: -. Publisher: San Diego [etc.]: Academic Press, cop. 1998  (Library)
  • Optical Imaging and Microscopy [Recurso electrónico] : Techniques and Advanced Systems. Edition: 2. Author: Török, Peter. Publisher: Berlin, Heidelberg : Springer-Verlag, 2007  (Library)
  • Técnicas en histología y biología celular. Edition: 2ª ed. Author: Montuenga Badía, Luis. Publisher: Elsevier-Masson, 2014  (Library)
  • Electron microscopy: principles and techniques for biologists. Edition: 2nd ed. Author: Bozzola, John J.. Publisher: Boston: Jones and Bartlett, cop. 1999  (Library)
  • Microscopy, immunohistochemistry, and antigen retrieval methods: for light and electron microscopy. Edition: -. Author: Hayat, M. A.. Publisher: New York [etc.]: Kluwer Academic Plenum Publishers, cop. 2002  (Library)
  • Culture of animal cells : a manual of basic technique and specialized applications. Edition: 6th ed. Author: Freshney, R. Ian. Publisher: Hoboken, NJ : Wiley-Blackwell, cop. 2010  (Library)
  • Basic cell culture : a practical approach. Edition: 2nd. ed., reimpr. Author: -. Publisher: Oxford ; New York : Oxford University, 2006  (Library)
  • Animal cell culture methods. Edition: -. Author: -. Publisher: San Diego [etc.]: Academic Press, cop. 1998  (Library)
  • Optical Imaging and Microscopy [Recurso electrónico] : Techniques and Advanced Systems. Edition: 2. Author: Török, Peter. Publisher: Berlin, Heidelberg : Springer-Verlag, 2007  (Library)
  • Técnicas en histología y biología celular. Edition: 2ª ed. Author: Montuenga Badía, Luis. Publisher: Elsevier-Masson, 2014  (Library)
  • Electron microscopy: principles and techniques for biologists. Edition: 2nd ed. Author: Bozzola, John J.. Publisher: Boston: Jones and Bartlett, cop. 1999  (Library)
  • Microscopy, immunohistochemistry, and antigen retrieval methods: for light and electron microscopy. Edition: -. Author: Hayat, M. A.. Publisher: New York [etc.]: Kluwer Academic Plenum Publishers, cop. 2002  (Library)
  • Culture of animal cells : a manual of basic technique and specialized applications. Edition: 6th ed. Author: Freshney, R. Ian. Publisher: Hoboken, NJ : Wiley-Blackwell, cop. 2010  (Library)
  • Basic cell culture : a practical approach. Edition: 2nd. ed., reimpr. Author: -. Publisher: Oxford ; New York : Oxford University, 2006  (Library)
  • Animal cell culture methods. Edition: -. Author: -. Publisher: San Diego [etc.]: Academic Press, cop. 1998  (Library)
ADDITIONAL BOOKLIST:
  • Tissue Engineering III: Cell - Surface Interactions for Tissue Culture [Recurso electrónico]. Edition: -. Author: Kasper, Cornelia. Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg, 2012  (Library)
  • http://www.nobelprize.org/educational/physics/microscopes/tem/tem.html. Edition: -. Author: -  (Library)
  • ◾ http://micro.magnet.fsu.edu/primer/virtual/confocal/index.html.. Edition: -. Author: -  (Library)
  • ◾ http://www.microscopyu.com/tutorials/java/phasecontrast/opticaltrain/index.html. Edition: -. Author: -  (Library)
  • http://www.olympusconfocal.com/java/confocalvswidefield/index.html. Edition: -. Author: -  (Library)
  • http://rsbweb.nih.gov/ij/. Edition: -. Author: -  (Library)
  • http://imagej.nih.gov/ij/. Edition: -. Author: -  (Library)
  • Cell culture technology for pharmaceutical and cell-based therapies. Edition: -. Author: -. Publisher: Boca Ratón: Taylor & Francis, 2006  (Library)
  • Tissue Engineering III: Cell - Surface Interactions for Tissue Culture [Recurso electrónico]. Edition: -. Author: Kasper, Cornelia. Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg, 2012  (Library)
  • http://www.nobelprize.org/educational/physics/microscopes/tem/tem.html. Edition: -. Author: -  (Library)
  • ◾ http://micro.magnet.fsu.edu/primer/virtual/confocal/index.html.. Edition: -. Author: -  (Library)
  • ◾ http://www.microscopyu.com/tutorials/java/phasecontrast/opticaltrain/index.html. Edition: -. Author: -  (Library)
  • http://www.olympusconfocal.com/java/confocalvswidefield/index.html. Edition: -. Author: -  (Library)
  • http://rsbweb.nih.gov/ij/. Edition: -. Author: -  (Library)
  • http://imagej.nih.gov/ij/. Edition: -. Author: -  (Library)
  • Cell culture technology for pharmaceutical and cell-based therapies. Edition: -. Author: -. Publisher: Boca Ratón: Taylor & Francis, 2006  (Library)
  • Tissue Engineering III: Cell - Surface Interactions for Tissue Culture [Recurso electrónico]. Edition: -. Author: Kasper, Cornelia. Publisher: Berlin, Heidelberg : Springer Berlin Heidelberg, 2012  (Library)
  • http://www.nobelprize.org/educational/physics/microscopes/tem/tem.html. Edition: -. Author: -  (Library)
  • ◾ http://micro.magnet.fsu.edu/primer/virtual/confocal/index.html.. Edition: -. Author: -  (Library)
  • ◾ http://www.microscopyu.com/tutorials/java/phasecontrast/opticaltrain/index.html. Edition: -. Author: -  (Library)
  • http://www.olympusconfocal.com/java/confocalvswidefield/index.html. Edition: -. Author: -  (Library)
  • http://rsbweb.nih.gov/ij/. Edition: -. Author: -  (Library)
  • http://imagej.nih.gov/ij/. Edition: -. Author: -  (Library)
  • Cell culture technology for pharmaceutical and cell-based therapies. Edition: -. Author: -. Publisher: Boca Ratón: Taylor & Francis, 2006  (Library)
7. VIRTUAL / CLASSROOM TEACHING SCENARIO

Teaching methodology

The theory classes in a large group will be carried out 100% in person if there are classrooms with enough space to maintain the safety distance. If this is not the case, they will be carried out semi-attendance at 50% and alternate. Half of the large group will attend a given class in person and the rest will attend via online videoconference, and in the next class, attendance is alternated.

The practices will be carried out in the laboratory in a small group with 100% presential attendance, if space is available that allows the safety distance to be maintained. If this is not the case, they will be carried out in a semi-presential manner at 50% and alternately, transmitting the session to non-attendees by videoconference. After each session and outside the laboratory, students must answer a questionnaire related to the corresponding practice.

The tutorials, individual or collective, will be carried out synchronously by videoconference or asynchronously through the Virtual Teaching forum.

  Evaluation systems

The criteria and evaluation systems of presential scenario are maintained, both in the ordinary and extraordinary convocatory.

  Means

Virtual teaching platform

Google-meet

8. VIRTUAL TEACHING SCENARIO

Teaching methodology

The large group theory classes will be held via synchronous online videoconference.

The laboratory practice sessions will be replaced by video demonstrations, directed to the small group by videoconference and tutored by the teaching staff. The videos will reflect the development of the experimental protocols proposed in the subject. The students will receive information and feasible results with the experimentation carried out in the demonstrations, which they will have to manage to respond to a series of exercises that must be delivered to the teaching staff for evaluation. The means for the delivery of exercises will be the Virtual Teaching platform.

The tutorials, individual or collective, will be carried out synchronously by videoconference or asynchronously through the Virtual Teaching forum.

Evaluation systems

The criteria and evaluation systems of the presential scenario are maintained, both in the ordinary and extraordinary convocatory.

Means

Virtual teaching platform

Google-meet

Online videos of theory master classes.

DATA PROTECTION CLAUSE (on line exams)

Institution in charge of data processing: Universidad de Jaén, Campus Las Lagunillas, s/n, 23071 Jaén

Data Protection Delegate: dpo@ujaen.es

Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Jaén to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.

Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.

Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Jaén has signed the corresponding data access contracts.

Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.

Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es

CLASS RECORDING CLAUSE PERSONAL DATA PROTECTION

Person in charge: Universidad de Jaén, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es

Data protection delegate (DPO): TELEFÓNICA, S.A.U. ; Email: dpo@ujaen.es

Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching

Period for record storage: Images will be kept during legal term according to regulations in force

Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents

Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality

Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es