Universidad de Jaén

Fundaments of proteomics: Protein folding, sorting, turnover, maturation, degradation and protein-ligand interactions.

Techniques and methods of sample preparation, separation (2-DE, HPLC-MS, other), identification and quantification in proteomics. Protein microarrays. ProteoRed.

Mass espectrometry in proteomics.

Applications of proteomis in the diagnostic, development of drugs, agriculture, industry.

Laboratory Practice: Effects of starvation on rat hepatic proteome by 2D SDS-PAGE. Sample preparation. 2-DE.Staining and analysis of proteins. Introduction to PDQest ^R (BioRad Laboratories). MS analysis. Identification of proteins and functional proteomics. Introduction to ProteoRed, the Spanish Network for Proteomics. Mass spectrometry equipment in the University of Jaén. Equipment and techniques for protein characterization in the Department of Experimental Biology of the University of Jaén.

An introduction to structural genomics: genome sequencing and phylogenetic structure.

An intoriduction to funtional genomics: tools for high-throughput gene expression and gene annotation.

Data Bases in Molecular Genetics:

1. Nucleotides: EMBL and Genbank (including dbGSS, dbSTS, dbSNP).
2. Proteins: NCBI, Expasy, EMBL; NCBI, RELIBASE, 3Dee.
3. Human gene project (Sanger), and other sequenced genomes (S. cerevisiae, B. subtilis, A. Thaliana, etc).
4. References (PubMed).
5. OMIM (On line Mendelian Inheritance in Man).

DNA and protein sequencing and analysis:

Training in automatical DNA sequencing ADN en secuenciados automático. Sequence analysis (BLAST, FASTA, CLUSTAL, seqaid, DNAsis, GCG).

Other Bioinformatic tools in Molecular Genetics:

1. Gene searching (Genscan, BCM search launcher).
2. PCR (oligo, Melting).
3. ARN (Fold).
4. Proteins and motifs (EMBOSS, Prosite, Expasy).
5. GWAS tools

Data analysis and modelling:

1. Microarrays data analysis. (R, NIH DAVID).
2. Data integration: multivariate analysis and data mining.
3. Molecular networks.
4. Systems Biotechnology.
5. Systems Biology and Systems Dynamics.

General methodology:

1) Academic sessions (20 h in presence of professor),

2) Laboratory sessions (15 h)

3) Practical class with computer adds (23 h)

3) Oral presentations (2 h)

  Course description and teaching methodology for PATIE student:

1) Lecture (Theory): Tutorial session, supply of press material, proposal of activities and exercises in English (in presence of professor or virtual): 1 h per week

2) Lecture (Practice): Tutorial session, supply of press material (in presence of professor or virtual): 1 h per week

c) Seminars: Supply of press material to prepare oral presentation: 1 h per week

d) Homework: 4 h per week

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

Assessment methodology:

Evaluation of attendance, involvement, and academic progress in: 1) theoric activities, 2) laboratory practice, 3) computer practice, 4) other activities.

The sum of the marks obtained in all of these aspects will be the end global mark. If the subject is not passed at Ordinary I  announcement, marks of the parts passed will be considered for Extraordinary II announcement. In this Extraordinary II announcement the mark corresponding to attendance and involving can be substituted by a written evaluation exam.

1- METHODOLOGY AND TRAINING ACTIVITIES

In this scenary, there are no changers with regards to the original course syllabus. If the face-to-face activities were not possible, it will be substituted by online activities.

General methodology:

1) Academic sessions (20 h in presence of professor),

2) Laboratory sessions (15 h)

3) Practical class with computer adds (23 h)

3) Oral presentations (2 h)

   Course description and teaching methodology for PATIE student:

1) Lecture (Theory): Tutorial session, supply of press material, proposal of activities and exercises in English (in presence of professor or virtual): 1 h per week

2) Lecture (Practice): Tutorial session, supply of press material (in presence of professor or virtual): 1 h per week

c) Seminars: Supply of press material to prepare oral presentation: 1 h per week

d) Homework: 4 h per week

2- EVALUATION SYSTEM

There are no changes with regard to the original course syllabus.

3- RESOURCES AND FACILITIES

• University of Jaén official e-mail addresses
• Online UJA platform
• Google Form
• Google Meet
• Each other set up for this effect

1- METHODOLOGY AND TRAINING ACTIVITIES

In this scenary, face-to-face activities will be changed by online activities.

General methodology:

1) Academic sessions (20 h in presence of professor),

2) Laboratory sessions (15 h)

3) Practical class with computer adds (23 h)

3) Oral presentations (2 h)

Course description and teaching methodology for PATIE student:

1) Lecture (Theory): Tutorial session, supply of press material, proposal of activities and exercises in English (in presence of professor or virtual): 1 h per week

2) Lecture (Practice): Tutorial session, supply of press material (in presence of professor or virtual): 1 h per week

c) Seminars: Supply of press material to prepare oral presentation: 1 h per week

d) Homework: 4 h per week

2- EVALUATION SYSTEM

There are no changes with regard to the original course syllabus, only with the exception that, in the ordinary and extraordinary announcement, the written exam will be online using the software and support recommended by the University of Jaén and following the regulations approved to that effect.

3- RESOURCES AND FACILITIES

• University of Jaén official e-mail addresses
• Online UJA platform
• Google Form
• Google Meet
• Each other set up for this effect

Institution in charge of data processing: Universidad de Jaén, Campus Las Lagunillas, s/n, 23071 Jaén

Data Protection Delegate: dpo@ujaen.es

Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Jaén to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.

Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.

Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Jaén has signed the corresponding data access contracts.

Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.

Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es

Person in charge: Universidad de Jaén, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es

Data protection delegate (DPO): TELEFÓNICA, S.A.U. ; Email: dpo@ujaen.es

Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching

Period for record storage: Images will be kept during legal term according to regulations in force

Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents

Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality

Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es