Universidad de Jaén

Theory Program

1. Preparation of materials and reagents. General methods for processing biological samples. Safety in the laboratory.

2. Spectrophotometry. The spectrophotometer: General concepts. Absorption spectrum. Lambert-Beer law. Spectrofluorimetry. Applications.

3. Chromatography: General concepts. Chromatography types. Column chromatographies. Applications.

4. Electrophoresis: General concepts. Electrophoresis types: acrylamide gel electrophoresis (PAGE) under native and denaturing conditions (SDS-PAGE), and agarose gel electrophoresis. Applications.

5. Cell culture. Necessary equipment. Culture media and optimization. pH control. Techniques for cell cultures. Contamination.

6. Plant culture under controlled conditions.In vitro culture of plants. Micropropagation. Organogenesis and embryogenesis. Hydroponics. Types. Substrates used.

7. Processing of samples for microscopic observation. Fixers. Microtomy and ultramicrotomy. Light and electron microscopy.

8. Tissue staining techniques and observation methods. Composition and types of dyes. Mechanisms of action. Types of staining. Stain for electron microscopy. Cytological methods. Special staining techniques.

Lessons 1-4 will be taught by the Biochemistry and Molecular Biology Area, lessons 5-8 will be taught by the Plant Physiology Area.

Practice Program

1. Identification and handling of general material and equipment of laboratory. Preparation of reagents and buffers. pH determination.

2. Processing of biological samples. Homogenization and differential centrifugation.

3. Spectrophotometry. Absorption spectrum of a substance. relationship between concentration and absorbance.

4. Flat chromatography and gel filtration.

5. Agarose gel and polyacrylamide gel electrophoresis (PAGE).

6. Preparation of media for in vitro culture of plants I. Preparation of stock solutions. MS and DKW media.

7. Preparation of media for in vitro culture of plants II. Specific media for callus culture, embryo rescue and organogenesis in various plant tissues.

8. Growing in vitro cultured callus and vegetative micropropagation of mature embryos. Management of Neubauer chamber and cell counting technique.

9. Processing plant and animal tissue samples I. Live tissues: vital dyes and optical microscope observation.

10. Preparation of plant and animal tissue sections II. Fixation, embedding, microtomy, staining, assembly and microscope observation.

Practices 1-5 will be taught by the Biochemistry and Molecular Biology Area, Practices 6-10 will be taught by the Plant Physiology Area.

Seminar Program

• Seminar 1: Resolution of problems using different concentration units. Theoretical calculations for the preparation of buffer solutions.
  
• Seminar 2: Review of acquired knowledge related to "in vitro" cultures and optical and electronic microscopy.

These contents connect with the United Nations sustainable development goals (SDGs). The eminently technical and practical character of this subject will reinforce SDGs 3, 6 and 9. The critical spirit, basic for achieving SDG 4, will be fostered.

The course consists of 16 sessions of lectures in large group (1 hour each). In the first 8 sessions, topics from 1 to 4 lessons will be explained , and in the following 8 sessions, topics from 5 to 8 lessons will be taught.

In addition, students must attend to 10 sessions of laboratory practices in small group (4 hours each). After each session, students must submit a questionnaire to the teacher where calculations and results obtained in the corresponding session will be collected. Five Practices will be taught by the Biochemistry and Molecular Biology Area and the other five sessions will be taught by the Plant Physiology Area.

And finally, 2 seminars (1 hour each) will be taught in the classroom in small group in order to solve activities related to the theoretical part of the course. 

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

The theoretical exam will have two parts corresponding to the lessons 1-4 and 5-8. It will be necessary to have a minimum calification of 5 points in each part of the theorical exam in order to consider the califications obtained in the other activities carried out in the subject.

Attendance to Laboratory classes and the completion of the Practice Notebook are mandatory and indispensable to pass the course. The calification of the Practice Notebook will be a 10% of the overall rating of the subject and the practical exam will represent a 30%.

The practical exam will have two parts corresponding to the practices 1-5 and 6-10. It will be necessary a minimum calification of 5 points in each part of the practical exam in order to consider them to calculate the final calification of the subject.

If the subject is not approved as a whole in the Ordinary call 1, the grades of the exams with more than 5 points will be saved only until the Extraordinary call 2. In this second call, students will repeat the exams of the failed parts. After this last call, students who do not pass all the exams of the subject (more than 5 points of 10) will have to take it again the following year.

1.  COURSE DESCRIPTION AND TEACHING METHODOLOGY

In
the event that the capacity of laboratories would not
allow the access of the entire groups, they would be subdivided
into two subgroups and 2 options would be applied:
Option 1. Practices 1-5.
Rotation of the subgroups and retransmission of the practical
classes to the rest of the group as long as the necessary
audiovisual means are available.
Option 2. Practices 6-10.
The 4 hours assigned to each practical session would be subdivided
into two 2-hour bands. One half of the small group would attend the
first time slot and the other half of the subgroup the second.
The schedule published on the FCC Experimentales website will be
followed.
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
       
        

 2.  ASSESSMENT METHODOLOGY

  Ordinary Convocatory

If the course is not approved in the Ordinary 1 convocatory, the marks of the exams passed will be saved only for the Extraordinary 2. If students don´t pass the final exams (more than 5 point of 10), they will have to repeat all the aspects to be evaluated again in the next year.

Extraordinary convocatory

 3.  RESOURCES

• ILIAS platform for virtual teaching at the University of Jaén to  provide teaching materials to students.
• Email from the UJA to send notices to the students and to attend asynchronous individual tutorials.
• Bibliographic resources from the Library of the University of Jaén ( https://www.ujaen.es/servicios/biblio ). 

In
the event that the socio-sanitary situation forces to
carry out teaching activities in a non-face-to-face format, the
following resources will be used, in addition to the above:

• Google Meet to conduct teaching videoconferences and individual and group tutoring, all synchronously.
• Google Forms to carry out continuous assesment activities and exams.
• Platform for virtual teaching at the University of Jaén to carry out forms and exams.

1. COURSE DESCRIPTION AND TEACHING METHODOLOGY

2. ASSESSMENT METHODOLOGY

Ordinary Convocatory

If the course is not approved in the Ordinary 1 convocatory, the marks of the exams passed will be saved only for the Extraordinary 2. If students don´t pass the final exams, they will have to repeat all the aspects of the subject in the next year.

Extraordinary Convocatory

1. RESOURCES

Adaptation of teaching to the multimodal or mixed and totally online model will be carried out using the following resources:

• Google Meet to conduct teaching videoconferences and individual and group tutoring, all synchronously.
• Google Forms to carry out continuous assesment activities and exams.
• Platform for virtual teaching at the University of Jaén to carry out forms, exams and provide teaching materials to students.
• Email from the UJA to send notices to the students and to attend asynchronous individual tutorials.
• Bibliographic resources from the Library of the University of Jaén ( https://www.ujaen.es/servicios/biblio ). 

Institution in charge of data processing: Universidad de Jaén, Campus Las Lagunillas, s/n, 23071 Jaén

Data Protection Delegate: dpo@ujaen.es

Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Jaén to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.

Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.

Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Jaén has signed the corresponding data access contracts.

Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.

Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es

Person in charge: Universidad de Jaén, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es

Data protection delegate (DPO): TELEFÓNICA, S.A.U. ; Email: dpo@ujaen.es

Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching

Period for record storage: Images will be kept during legal term according to regulations in force

Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents

Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality

Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es