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Syllabus 2021-22 - 10211006 - Lab Methods and Instruments (Métodos e instrumentación de laboratorio)

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  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Grado en Biología
FACULTY: FACULTY OF EXPERIMENTAL SCIENCES
ACADEMIC YEAR: 2021-22
COURSE: Lab Methods and Instruments
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Lab Methods and Instruments
CODE: 10211006 ACADEMIC YEAR: 2021-22
LANGUAGE: English LEVEL: 2
ECTS CREDITS: 6.0 YEAR: 1 SEMESTER: PC
2. LECTURER BASIC INFORMATION
NAME: VALDERRAMA RODRÍGUEZ, RAQUEL
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 341 E-MAIL: ravalde@ujaen.es P: 953-212768
WEBSITE: ravalde@ujaen.es
ORCID: https://orcid.org/0000-0003-2253-0101
LANGUAGE: English LEVEL: 2
NAME: BEGARA MORALES, JUAN CARLOS
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 08 E-MAIL: jbegara@ujaen.es P: 953213058
WEBSITE: -
ORCID: -
LANGUAGE: English LEVEL: 2
3. CONTENT DESCRIPTION

Theory Program

1. Preparation of materials and reagents. General methods for processing biological samples. Safety in the laboratory.

2. Spectrophotometry. The spectrophotometer: General concepts. Absorption spectrum. Lambert-Beer law. Spectrofluorimetry. Applications.

3. Chromatography: General concepts. Chromatography types. Column chromatographies. Applications.

4. Electrophoresis: General concepts. Electrophoresis types: acrylamide gel electrophoresis (PAGE) under native and denaturing conditions (SDS-PAGE), and agarose gel electrophoresis. Applications.

5. Cell culture. Necessary equipment. Culture media and optimization. pH control. Techniques for cell cultures. Contamination.

6. Plant culture under controlled conditions.In vitro culture of plants. Micropropagation. Organogenesis and embryogenesis. Hydroponics. Types. Substrates used.

7. Processing of samples for microscopic observation. Fixers. Microtomy and ultramicrotomy. Light and electron microscopy.

8. Tissue staining techniques and observation methods. Composition and types of dyes. Mechanisms of action. Types of staining. Stain for electron microscopy. Cytological methods. Special staining techniques.

Lessons 1-4 will be taught by the Biochemistry and Molecular Biology Area, lessons 5-8 will be taught by the Plant Physiology Area.

Practice Program

1. Identification and handling of general material and equipment of laboratory. Preparation of reagents and buffers. pH determination.

2. Processing of biological samples. Homogenization and differential centrifugation.

3. Spectrophotometry. Absorption spectrum of a substance. relationship between concentration and absorbance.

4. Flat chromatography and gel filtration.

5. Agarose gel and polyacrylamide gel electrophoresis (PAGE).

6. Preparation of media for in vitro culture of plants I. Preparation of stock solutions. MS and DKW media.

7. Preparation of media for in vitro culture of plants II. Specific media for callus culture, embryo rescue and organogenesis in various plant tissues.

8. Growing in vitro cultured callus and vegetative micropropagation of mature embryos. Management of Neubauer chamber and cell counting technique.

9. Processing plant and animal tissue samples I. Live tissues: vital dyes and optical microscope observation.

10. Preparation of plant and animal tissue sections II. Fixation, embedding, microtomy, staining, assembly and microscope observation.

Practices 1-5 will be taught by the Biochemistry and Molecular Biology Area, Practices 6-10 will be taught by the Plant Physiology Area.

Seminar Program

Practical application of adquired knowledge to solving activities.

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

The course consists of 16 sessions of lectures in large group (1 our each). In the first 8 sessions, topics from 1 to 4 lessons will be explained , and in the following 8 sessions, topics from 5 to 8 lessons will be taught.

In addition, students must attend to 10 sessions of laboratory practices in small group (4 hours each). After each session, students must submit a questionnaire to the teacher where calculations and results obtained in the corresponding session will be collected. Five Practices will be taught by the Biochemistry and Molecular Biology Area and the other five sessions will be taught by the Plant Physiology Area.

And finally, 2 seminars (1 hour each) will be taught in the classroom in small group in order to solve activities related to the theoretical part of the course. 

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

The theoretical exam will have two parts corresponding to the lessons 1-4 and 5-8. It will be necessary to have a minimum calification of 5 points in each part of the theorical exam in order to consider the califications obtained in the other activities carried out in the subject.

Attendance to Laboratory classes and the completion of the Practice Notebook are mandatory and indispensable to pass the course. The calification of the Practice Notebook will be a 10% of the overall rating of the subject and the practical exam will represent a 30%.

The practical exam will have two parts corresponding to the practices 1-5 and 6-10. It will be necessary a minimum calification of 4 points in each part of the practical exam in order to consider them to calculate the final calification of the subject.

If the course is not passed in the Ordinary I convocatory, partial califications (activities and/or practices) will be saved only for the Extraordinary II. If students don´t pass the exams, they may repeat all the aspects to be evaluated in the subject in the next year.

6. BOOKLIST
MAIN BOOKLIST:
  • Plant tissue culture: theory and practice, a revised edition. Edition: -. Author: Bhojwani, S. S.. Publisher: Amsterdam [etc.]: Elsevier Science, 1996  (Library)
ADDITIONAL BOOKLIST:
  • Principles and techniques of biochemistry and molecular biology. Edition: 6th ed. Author: -. Publisher: Cambridge: Cambridge University Press, 2005  (Library)
  • At the bench: a laboratory navigator. Edition: Updated ed.. Author: Barker, Kathy, 1953-. Publisher: Cold Spring Harbor (New York): Cold Spring Harbor Laboratory Press, 2005.  (Library)
  • Plant tissue culture [Recurso electrónico] : techniques and experiments. Edition: 3rd ed. Author: Smith, Roberta H. Publisher: London ; Waltham, MA : Academic Press, ©2013  (Library)
7. VIRTUAL / CLASSROOM TEACHING SCENARIO

1.  COURSE DESCRIPTION AND TEACHING METHODOLOGY

Formation activities

Format (classroom/ online)

Teaching methodology description

16 Lecture sessions

Classroom

16 One-hour master class sessions taught  to the large group according to the Schedule published on the Faculty of Experimental Sciences website.

2 Solving problems/ exercises sessions

In the classroom

2 One-hour seminar sessions taught in the classroom by the teacher to the small groups according to the Schedule published on the Faculty of Experimental Sciences website.

10 Practical sessions in specialized labs

Classroom

10 Four-hours practical sessions in teaching laboratories.

In
the event that the capacity of laboratories would not
allow the access of the entire groups, they would be subdivided
into two subgroups and 2 options would be applied:
Option 1. Practices 1-5.
Rotation of the subgroups and retransmission of the practical
classes to the rest of the group as long as the necessary
audiovisual means are available.
Option 2. Practices 6-10.
The 4 hours assigned to each practical session would be subdivided
into two 2-hour bands. One half of the small group would attend the
first time slot and the other half of the subgroup the second.
The schedule published on the FCC Experimentales website will be
followed.
       
       
       
       
       
        

Collective tutoring

Classroom

Collective tutoring to the large group if it is necessary

 

 2.  ASSESSMENT METHODOLOGY

  Ordinary Convocatory

Assessment probe

Format (classroom/ online synchronous or asynchronous)

Description

Percentage

Assessment of theoretical knowledge

Classroom

The theory exam will consist of two parts corresponding to the blocks of lessons 1-4 and 5-8. It will be necessary to obtain a minimum grade of 5 points in each part of the theory exam in order to consider the grade obtained in the rest of activities carried out in the subject.

50%

Assessment of the activities proposed in the seminar classes

Presentation of activities within the established period

The activities carried out in person in the seminar clases will be graded. Students will present the activities to the teacher  for correction.

10%

Laboratory practices

Presentation of practice questionnaires in electronic format within the established period

 

 

 Classroom

Attendance at all the practice sessions as well as the presentation of the practice questionnaires are mandatory and essential to pass the course.

The qualification of the practice quiestionnaires will suppose 10% of the global qualification of the subject and the practical exam will represent 30%.

The practice exam will consist of two parts corresponding to practice lessons 1-5 and 6-10. A mínimum grade of 4 points is required in each part of the practical exam in order to consider these marks together with those obtained in the seminar clases, practical questionnaires and theory exams to carry out the weighted calculation of the final grade.

40%

If the course is not approved in the Ordinary 1 convocatory, the marks of the complete passed parts will be saved only for the Extraordinary 2. If students don´t pass the final exams, they will have to repeat all the aspects to be evaluated in the subject in the next year.

Extraordinary convocatory

Assessment probe

Format (classroom/ online synchronous or asynchronous)

Description

Percentage

Assessment of theoretical knowledge

Classroom

The same as Ordinary Convocatory

50%

Assessment of the activities proposed in the seminar classes

Presentation of activities  within the established period

The same as Ordinary Convocatory

10%

Laboratory practices

Presentation of practice questionnaires  within the established period

 

 

 

Classroom

The same as Ordinary Convocatory

 

40%

 3.  RESOURCES

  • ILIAS platform for virtual teaching at the University of Jaén to  provide teaching materials to students.
  • Email from the UJA to send notices to the students and to attend asynchronous individual tutorials.
  • Bibliographic resources from the Library of the University of Jaén ( https://www.ujaen.es/servicios/biblio ). 

 

In
the event that the socio-sanitary situation forces to
carry out teaching activities in a non-face-to-face format, the
following resources will be used, in addition to the above:

  • Google Meet to conduct teaching videoconferences and individual and group tutoring, all synchronously.
  • Google Forms to carry out continuous assesment activities and exams.
  • Platform for virtual teaching at the University of Jaén to carry out forms and exams.

8. VIRTUAL TEACHING SCENARIO

  1. COURSE DESCRIPTION AND TEACHING METHODOLOGY

Formation activities

Format (classroom/ online)

Teaching methodology description

16 Lecture sessions

Online

16 One-hour master class sessions taught synchronously and online via videoconference by the teacher to the large group according to the Schedule published on the Faculty of Experimental Sciences website.

2 Solving problems/ exercises sessions

Online

2 One-hour seminar sessions taught synchronously and online via videoconference by the teacher to the small groups according to the Schedule published on the Faculty of Experimental Sciences website.

10 Practical sessions in specialized labs

Online

10 Four-hours practical sessions taught synchronously and online via videoconference by the teacher to the small groups according to the Schedule published on the Faculty of Experimental Sciences website. During those 4 hours, in addition to the explanation of each practical session, the students will be provided with the numerical results and images necessary to complete, also during that time, the questions contained in the Practice Guide.

Collective tutoring

Online

If necessary, collective tutoring to the large group will be online (synchronous and asynchronously). 

2. ASSESSMENT METHODOLOGY

Ordinary Convocatory

Assessment probe

Format (classroom/ online synchronous or asynchronous)

Description

Percentage

Assessment of theoretical knowledge

Online synchronous

Online forms made up of test questions and short answer questions using the Google Forms application and/or the UJA  platform. The theory exam will consist of two parts corresponding to the blocks of lessons 1-4 and 5-8. It will be necessary to obtain a minimum grade of 5 points in each part of the theory exam in order to consider the grade obtained in the rest of activities carried out in the subject.

50%

Assessment of the activities proposed in the online seminar classes

Presentation of activities in electronic format within the established period

The activities carried out in the online seminar classes will be graded. Students will present the activities to the teacher through the platform for correction.

10%

Online laboratory practices

 

Presentation of practice questionnaires in electronic format within the established period

 

Practice exam in synchronous online format

 

Attendance to all video calls established for online practice sessions as well as the presentation of the practice questionnaires are mandatory and essential to pass the course.

The qualification of the practice quiestionnaires will suppose 10% of the global qualification of the subject and the practical exam will represent 30%.

The practice exam will consist of two parts corresponding to practice lessons 1-5 and 6-10. A mínimum grade of 4 points is required in each part of the practical exam in order to consider these marks together with those obtained in the seminar clases, practical questionnaires and theory exams to carry out the weighted calculation of the final grade.

40%

If the course is not approved in the Ordinary 1 convocatory, the marks of the complete passed parts will be saved only for the Extraordinary 2. If students don´t pass the final exams, they will have to repeat all the aspects to be evaluated in the subject in the next year.

 

Extraordinary Convocatory

Assessment probe

Format (classroom/ online synchronous or asynchronous)

Description

Percentage

Assessment of theoretical knowledge

Online synchronous

The same as Ordinary Convocatory

50%

Assessment of the activities proposed in the online seminar classes

Presentation of activities in electronic format within the established period

The same as Ordinary Convocatory

10%

Online laboratory practices

 

Presentation of practice questionnaires in electronic format within the established period

 

Practice exam in synchronous online format

 

The same as Ordinary Convocatory

40%

 

  1. RESOURCES

Adaptation of teaching to the multimodal or mixed and totally online model will be carried out using the following resources:

  • Google Meet to conduct teaching videoconferences and individual and group tutoring, all synchronously.
  • Google Forms to carry out continuous assesment activities and exams.
  • Platform for virtual teaching at the University of Jaén to carry out forms, exams and provide teaching materials to students.
  • Email from the UJA to send notices to the students and to attend asynchronous individual tutorials.
  • Bibliographic resources from the Library of the University of Jaén ( https://www.ujaen.es/servicios/biblio ). 

 

DATA PROTECTION CLAUSE (on line exams)

Institution in charge of data processing: Universidad de Jaén, Campus Las Lagunillas, s/n, 23071 Jaén

Data Protection Delegate: dpo@ujaen.es

Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Jaén to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.

Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.

Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Jaén has signed the corresponding data access contracts.

Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.

Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es

CLASS RECORDING CLAUSE PERSONAL DATA PROTECTION

Person in charge: Universidad de Jaén, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es

Data protection delegate (DPO): TELEFÓNICA, S.A.U. ; Email: dpo@ujaen.es

Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching

Period for record storage: Images will be kept during legal term according to regulations in force

Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents

Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality

Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es