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Syllabus 2020-21 - 76712005 - Protein and Metabolite Purification and Analysis Techniques (Técnicas de purificación y análisis de proteínas y metabolitos)
- Level 1: Tutorial support sessions, materials and exams in this language
- Level 2: Tutorial support sessions, materials, exams and seminars in this language
- Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: | Máster Univ. en Biotecnología y biomedicina (76712005) |
FACULTY: | Centro de Estudios de Postgrado |
DEGREE: | Doble Máster en Biotecnología y Biomedicina y MFPES (Profesorado) (77912002) |
FACULTY: | Centro de Estudios de Postgrado |
ACADEMIC YEAR: | 2020-21 |
COURSE: | Protein and Metabolite Purification and Analysis Techniques |
NAME: Protein and Metabolite Purification and Analysis Techniques | |||||
CODE: 76712005 (*) | ACADEMIC YEAR: 2020-21 | ||||
LANGUAGE: English | LEVEL: 2 | ||||
ECTS CREDITS: 6.0 | YEAR: 1 | SEMESTER: PC |
NAME: PEDRAJAS CABRERA, JOSÉ RAFAEL | ||
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL | ||
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR | ||
OFFICE NO.: B3 - 348 | E-MAIL: pedrajas@ujaen.es | P: 953213634 |
WEBSITE: https://www.ujaen.es/departamentos/bioexp/contactos/pedrajas-cabrera-jose-rafael | ||
ORCID: https://orcid.org/0000-0003-1998-0638 | ||
LANGUAGE: - | LEVEL: 1 | |
NAME: PERAGÓN SÁNCHEZ, JUAN | ||
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL | ||
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR | ||
OFFICE NO.: B3 - 356 | E-MAIL: jperagon@ujaen.es | P: 953212523 |
WEBSITE: www4.ujaen.es/~jperagon/ | ||
ORCID: https://orcid.org/0000-0002-7502-1119 | ||
LANGUAGE: - | LEVEL: 2 |
This subject offers theoretical and practical training in the following aspects:
- Structural and functional fundamentals of proteins.
- Methods of protein analysis and identification
- Kinetic characterization of enzymes.
- Preparation of samples for protein purification
- Protein production by recombinant DNA
- Chromatographic strategies for the purification of native and recombinant proteins.
- Applications with recombinant proteins
- Fundamentals of metabolomics
- Methods for the analysis of metabolites
Theoretical lessons taught by teachers with support of audiovisual resources.
Practical activities in the laboratory. Students should prepare reports of the results.
Performance of exercises by students.
Students may have notes, experimental protocols, bibliographic material, etc. contributed by the teachers through a virtual teaching platform.
Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support
- Attendance (10%)
- Active participation (10%)
- Technical skills and quality of laboratory report (80%)
- The handbook of metabonomics and metabolomics [Recurso electrónico] . Edition: -. Author: -. Publisher: Amsterdam ; Oxford : Elsevier, 2007. (Library)
- Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation. Edition: -. Author: B. Álvarez Sánchez, F. Priego Capote, M.D. Luque de Castro. Publisher: Trends Anal. Chem. 29 (2010) 120-127 (Library)
- Metabolomics analysis II. Preparation of biological samples prior to detection. Edition: -. Author: B. Álvarez Sánchez, F. Priego Capote, M.D. Luque de Castro. . Publisher: Trends Anal. Chem. 29 (2010) (Library)
- Metabolite identification and quantitation in LC-MS/MS-based metabolomics. Edition: -. Author: J.F. Xiao, B. Zhou, H.W. Ressom. Publisher: Trends Anal. Chem. 32 (2012) 1-14 (Library)
- Preparation of urine samples prior to targeted or untargeted metabolomics mass-spectrometry analysis. Edition: -. Author: -. Publisher: Trends Anal. Chem. 41 (2012) 75-85 (Library)
- Basic methods in protein: purification and analysis : a laboratory manual . Edition: -. Author: -. Publisher: Cold Spring Harbor : Cold Spring Harbor Laboratory, 2009 (Library)
- Protein purification techniques: a practical approach. Edition: 2nd ed. Author: -. Publisher: Oxford: Oxford University Press, 2001 (Library)
- Protein purification applications: a practical approach. Edition: 2nd ed. Author: -. Publisher: Oxford: Oxford University Press, 2001 (Library)
- Enzyme assays: a practical approach. Edition: -. Author: -. Publisher: Oxford [etc.]: Oxford University Press Press, 2002 (Library)
- Molecular and Cellular Enzymology [Recurso electrónico]. Edition: -. Author: Yon-Kahn, Jeannine. Publisher: Berlin, Heidelberg : Springer-Verlag Berlin Heidelberg, 2010. (Library)
- Advances in enzymology and related areas of molecular biology. Volume 76 . Edition: -. Author: -. Publisher: New York : John Wiley & Sons, 2009 (Library)
- Guide to protein purification.. Edition: 2ª ed. Author: -. Publisher: San Diego [etc.] : Academic Press, cop. 2009 (Library)
- Principles and techniques of biochemistry and molecular biology. Edition: 7th ed. Author: -. Publisher: New York : Cambridge University Press, 2013 (Library)
Theory classes in a large group will be held in person if there are classrooms with enough space to maintain a safe distance. Alternatively, half a large group will attend the class in person and the rest will attend via online video conference. In the next class, the half group alternates.
Practices will be held in the lab or in the computer room in a small group, if space is available to maintain a safe distance. Otherwise, the practices are carried out at 50% in person, transmitting the session to non-attendees by videoconference. After each session and outside the laboratory, students must answer a questionnaire related to the corresponding practice.
The tutorials, individual or collective, will be carried out synchronously by videoconference or asynchronously through the Virtual Teaching forum.
The evaluation system is the same as in point 7 of this guide, both in the ordinary and in the extraordinary call.
Means:
ILIAS virtual teaching platform
Google Meet
Large group theory classes will be conducted via online synchronous videoconferencing.
The laboratory practical sessions will be replaced by video demonstrations, directed to the small group by videoconference and tutored by the teaching staff. The videos will reflect the development of the experimental protocols proposed in the subject. The students will receive information and feasible results with the experimentation carried out in the demonstrations, which they will have to manage to respond to a series of exercises that must be handed over to the teachers for their evaluation. The delivery of exercises will be through the Virtual Teaching platform.
The evaluation system is the same as in point 7 of this guide, both in the ordinary and in the extraordinary call.
Means:
ILIAS virtual teaching platform
Google Meet
Institution in charge of data processing: Universidad de Jaén, Campus Las Lagunillas, s/n, 23071 Jaén
Data Protection Delegate: dpo@ujaen.es
Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Jaén to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.
Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.
Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Jaén has signed the corresponding data access contracts.
Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.
Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es
Person in charge: Universidad de Jaén, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es
Data protection delegate (DPO): TELEFÓNICA, S.A.U. ; Email: dpo@ujaen.es
Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching
Period for record storage: Images will be kept during legal term according to regulations in force
Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents
Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality
Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es