Guías docentes UJA

NAME: Lab Methods and Instruments
CODE: 10211006		ACADEMIC YEAR: 2020-21
LANGUAGE: English		LEVEL: 2
ECTS CREDITS: 6.0	YEAR: 1		SEMESTER: PC

NAME: VALDERRAMA RODRÍGUEZ, RAQUEL
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 341	E-MAIL: ravalde@ujaen.es	P: 953-212768
WEBSITE: ravalde@ujaen.es
ORCID: https://orcid.org/0000-0003-2253-0101
LANGUAGE: English	LEVEL: 2
NAME: BUENO GONZÁLEZ, MILAGROS
DEPARTMENT: U102 - BIOLOGIA ANIMAL, BIOL.VEGETAL Y ECOLOGIA
FIELD OF STUDY: 412 - FISIOLOGÍA VEGETAL
OFFICE NO.: B3 - 143	E-MAIL: mbueno@ujaen.es	P: 953-212140
WEBSITE: -
ORCID: https://orcid.org/0000-0002-3079-5380
LANGUAGE: English	LEVEL: 2
NAME: CARRERAS EGAÑA, ALFONSO MARÍA
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - B3-349	E-MAIL: acarrera@ujaen.es	P: 953212765
WEBSITE: http://www4.ujaen.es/~acarrera
ORCID: https://orcid.org/0000-0003-2312-6602
LANGUAGE: English	LEVEL: 2

Theory Program

1. Preparation of materials and reagents. General methods for processing biological samples. Safety in the laboratory.

2. Spectrophotometry. The spectrophotometer: General concepts. Absorption spectrum. Lambert-Beer law. Spectrofluorimetry. Applications.

3. Chromatography: General concepts. Chromatography types. Column chromatographies. Applications.

4. Electrophoresis: General concepts. Electrophoresis types: acrylamide gel electrophoresis (PAGE) under native and denaturing conditions (SDS-PAGE), and agarose gel electrophoresis. Applications.

5. Cell culture. Necessary equipment. Culture media and optimization. pH control. Techniques for cell cultures. Contamination.

6. Plant culture under controlled conditions.In vitro culture of plants. Micropropagation. Organogenesis and embryogenesis. Hydroponics. Types. Substrates used.

7. Processing of samples for microscopic observation. Fixers. Microtomy and ultramicrotomy. Light and electron microscopy.

8. Tissue staining techniques and observation methods. Composition and types of dyes. Mechanisms of action. Types of staining. Stain for electron microscopy. Cytological methods. Special staining techniques.

Lessons 1-4 will be taught by the Biochemistry and Molecular Biology Area, lessons 5-8 will be taught by the Plant Physiology Area.

Practice Program

1. Identification and handling of general material and equipment of laboratory. Preparation of reagents and buffers. pH determination.

2. Processing of biological samples. Homogenization and differential centrifugation.

3. Spectrophotometry. Absorption spectrum of a substance. relationship between concentration and absorbance.

4. Flat chromatography and gel filtration.

5. Agarose gel and polyacrylamide gel electrophoresis (PAGE).

6. Preparation of media for in vitro culture of plants I. Preparation of stock solutions. MS and DKW media.

7. Preparation of media for in vitro culture of plants II. Specific media for callus culture, embryo rescue and organogenesis in various plant tissues.

8. Growing in vitro cultured callus and vegetative micropropagation of mature embryos. Management of Neubauer chamber and cell counting technique.

9. Processing plant and animal tissue samples I. Live tissues: vital dyes and optical microscope observation.

10. Preparation of plant and animal tissue sections II. Fixation, embedding, microtomy, staining, assembly and microscope observation.

Practices 1-5 will be taught by the Biochemistry and Molecular Biology Area, Practices 6-10 will be taught by the Plant Physiology Area.

Seminar Program

Practical application of adquired knowledge to solving activities.

The course consists of 16 sessions of lectures in large group (1 our each). In the first 8 sessions, topics from 1 to 4 lessons will be explained , and in the following 8 sessions, topics from 5 to 8 lessons will be taught.

In addition, students must attend to 10 sessions of laboratory practices in small group (4 hours each). After each session, students must submit a questionnaire to the teacher where calculations and results obtained in the corresponding session will be collected. Five Practices will be taught by the Biochemistry and Molecular Biology Area and the other five sessions will be taught by the Plant Physiology Area.

And finally, 2 seminars (1 hour each) will be taught in the classroom in small group in order to solve activities related to the theoretical part of the course. Each area

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

The theoretical exam will have two parts corresponding to the lessons 1-4 and 5-8. It will be necessary to have a minimum calification of 5 points in each part of the theorical exam in order to consider the califications obtained in the other activities carried out in the subject.

Attendance to Laboratory classes and the completion of the Practice Notebook are mandatory and indispensable to pass the course. The calification of the Practice Notebook will be a 10% of the overall rating of the subject and the practical exam will represent a 30%.

The practical exam will have two parts corresponding to the practices 1-5 and 6-10. It will be necessary a minimum calification of 4 points in each part of the practical exam in order to consider them to calculate the final calification of the subject.

If the course is not passed in the Ordinary I convocatory, partial califications (activities and/or practices) will be saved only for the Extraordinary II. If students don´t pass the exams, they may repeat all the aspects to be evaluated in the subject in the next year.

It´s forbidden handling of mobile phones during classes (theory, seminars and practices).
It´s forbidden video recordings or taking photos during classes (theory, seminars and practices).
Students must write accurately, legibly and without spelling mistakes.

Failure of any of the above conditions may lead to failing the course.

MAIN BOOKLIST:

Plant tissue culture: theory and practice, a revised edition. Edition: -. Author: Bhojwani, S. S.. Publisher: Amsterdam [etc.]: Elsevier Science, 1996 (Library)

ADDITIONAL BOOKLIST:

Principles and techniques of biochemistry and molecular biology. Edition: 6th ed. Author: -. Publisher: Cambridge: Cambridge University Press, 2005 (Library)
At the bench: a laboratory navigator. Edition: Updated ed.. Author: Barker, Kathy, 1953-. Publisher: Cold Spring Harbor (New York): Cold Spring Harbor Laboratory Press, 2005. (Library)
Plant tissue culture [Recurso electrónico] : techniques and experiments. Edition: 3rd ed. Author: Smith, Roberta H. Publisher: London ; Waltham, MA : Academic Press, ©2013 (Library)

1. COURSE DESCRIPTION AND TEACHING METHODOLOGY

Formation activities	Format (classroom/ online)	Teaching methodology description
16 Lecture sessions	Online	16 One-hour master class sessions taught synchronously and online via videoconference by the teacher to the large group according to the Schedule published on the Faculty of Experimental Sciences website.
2 Solving problems/ exercises sessions	100% In the classroom	2 One-hour seminar sessions taught in the classroom by the teacher to the small groups according to the Schedule published on the Faculty of Experimental Sciences website.
10 Practical sessions in specialized labs	50% In the classroom	Option 1. 10 Four-hours practical sessions in teaching laboratories applying a 50% rotation of the small groups of students. Retransmision of the practical sessions to the rest of the group will be conditioned to the equipment of the teaching laboratorios with the necessary audiovisual media. The Schedule published on the Faculty of Experimental Sciences website will be followed. Option 2. 10 Two-hours practical sessions in teaching laboratories. To carry out this option, the 4 hour time slot would be subdivided into 2-hour slots. Each half of the small group would attend to different slots according to the Schedule published on the Faculty of Experimental Sciences website. The teaching hours would be completed with: 1) virtual materials that the student must view and study asynchronously before attending each practical session, 2) asynchronous, and previous to the practical sessions, answering of online forms on issues related to the virtual materials, and 3) asynchronous, and outside the teaching lab, completion of the questionnaires collected in the Practice Guide after attending each practical session.
Collective tutoring	Online	Collective tutoring to the large group will be online (synchronous and asynchronously).

2. ASSESSMENT METHODOLOGY

Ordinary Convocatory

Assessment probe	Format (classroom/ online synchronous or asynchronous)	Description	Percentage
Assessment of theoretical knowledge	Online synchronous	Online forms made up of test questions and short answer questions using the Google Forms application and/or the UJA ILIAS platform. The theory exam will consist of two parts corresponding to the blocks of lessons 1-4 and 5-8. It will be necessary to obtain a minimum grade of 5 points in each part of the theory exam in order to consider the grade obtained in the rest of activities carried out in the subject.	50%
Assessment of the activities proposed in the seminar classes	Presentation of activities in electronic format within the established period	The activities carried out in person in the seminar clases will be graded. Students will present the activities to the teacher through the ILIAS platform for correction.	10%
Laboratory practices	Presentation of practice questionnaires in electronic format within the established period Practice exam in synchronous online format	Attendance at all the practice sessions as well as the presentation of the practice questionnaires are mandatory and essential to pass the course. For option 1 of practical teaching. The qualification of the practice quiestionnaires will suppose 10% of the global qualification of the subject and the practical exam will represent 30%. The practice exam will consist of two parts corresponding to practice lessons 1-5 and 6-10. A mínimum grade of 4 points is required in each part of the practical exam in order to consider these marks together with those obtained in the seminar clases, practical questionnaires and theory exams to carry out the weighted calculation of the final grade. In option 2 of practical teaching, in addition to the above, the asynchronous response, and prior to attending the practical sessions, of the online forms on issues related to virtual materials will be taken into account. Based on this, the percentages of the parts to be evaluated would be modified as follows: practice questionnaires 10%, online forms 10%, and practice exam 20%.	40%

If the course is not approved in the Ordinary 1 convocatory, the marks of the complete passed parts will be saved only for the Extraordinary 2. If students don´t pass the final exams, they will have to repeat all the aspects to be evaluated in the subject in the next year.

Extraordinary convocatory

Assessment probe	Format (classroom/ online synchronous or asynchronous)	Description	Percentage
Assessment of theoretical knowledge	Online synchronous	Online forms made up of test questions and short answer questions using the Google Forms application and/or the UJA ILIAS platform. The theory exam will consist of two parts corresponding to the blocks of lessons 1-4 and 5-8. It will be necessary to obtain a minimum grade of 5 points in each part of the theory exam in order to consider the grade obtained in the rest of activities carried out in the subject.	50%
Assessment of the activities proposed in the seminar classes	Presentation of activities in electronic format within the established period	The activities carried out in person in the seminar classes will be graded. Students will present the activities to the teacher through the ILIAS platform for correction.	10%
Laboratory practices	Presentation of practice questionnaires in electronic format within the established period Practice exam in synchronous online format	Attendance at all the practice sessions as well as the presentation of the practice questionnaires are mandatory and essential to pass the course. For option 1 of practical teaching. The qualification of the practice quiestionnaires will suppose 10% of the global qualification of the subject and the practical exam will represent 30%. The practice exam will consist of two parts corresponding to practice lessons 1-5 and 6-10. A mínimum grade of 4 points is required in each part of the practical exam in order to consider these marks together with those obtained in the seminar clases, practical questionnaires and theory exams to carry out the weighted calculation of the final grade. In option 2 of practical teaching, in addition to the above, the asynchronous response, and prior to attending the practical sessions, of the online forms on issues related to virtual materials will be taken into account. Based on this, the percentages of the parts to be evaluated would be modified as follows: practice questionnaires 10%, online forms 10%, and practice exam 20%.	40%

3. RESOURCES

Adaptation of teaching to the multimodal or mixed and totally online model will be carried out using the following resources:

Google Meet to conduct teaching videoconferences and individual and group tutoring, all synchronously.
Google Forms to carry out continuous assesment activities and exams.
ILIAS platform for virtual teaching at the University of Jaén to carry out forms, exams and provide teaching materials to students.
Email from the UJA to send notices to the students and to attend asynchronous individual tutorials.
Bibliographic resources from the Library of the University of Jaén ( https://www.ujaen.es/servicios/biblio ).

COURSE DESCRIPTION AND TEACHING METHODOLOGY

Formation activities	Format (classroom/ online)	Teaching methodology description
16 Lecture sessions	Online	16 One-hour master class sessions taught synchronously and online via videoconference by the teacher to the large group according to the Schedule published on the Faculty of Experimental Sciences website.
2 Solving problems/ exercises sessions	Online	2 One-hour seminar sessions taught synchronously and online via videoconference by the teacher to the small groups according to the Schedule published on the Faculty of Experimental Sciences website.
10 Practical sessions in specialized labs	Online	10 Sesiones prácticas de 4 horas de duración impartidas por el profesorado de manera síncroma y online mediante videoconferencia a los pequeños grupos según el horario publicado en la página web de la FCC Experimentales. Durante esas 4 horas, además de realizarse la explicación de cada sesión práctica, se facilitará al estudiantado los resultados numéricos e imágenes necesarias para que puedan cumplimentar, también durante ese tiempo, las cuestiones recogidas en el Guion de Prácticas. 10 Four-hours practical sessions taught synchronously and online via videoconference by the teacher to the small groups according to the Schedule published on the Faculty of Experimental Sciences website. During those 4 hours, in addition to the explanation of each practical session, the students will be provided with the numerical results and images necessary to complete, also during that time, the questions contained in the Practice Guide.
Collective tutoring	Online	Collective tutoring to the large group will be online (synchronous and asynchronously).

2. ASSESSMENT METHODOLOGY

Ordinary Convocatory

Assessment probe	Format (classroom/ online synchronous or asynchronous)	Description	Percentage
Assessment of theoretical knowledge	Online synchronous	Online forms made up of test questions and short answer questions using the Google Forms application and/or the UJA ILIAS platform. The theory exam will consist of two parts corresponding to the blocks of lessons 1-4 and 5-8. It will be necessary to obtain a minimum grade of 5 points in each part of the theory exam in order to consider the grade obtained in the rest of activities carried out in the subject.	50%
Assessment of the activities proposed in the online seminar classes	Presentation of activities in electronic format within the established period	The activities carried out in the online seminar classes will be graded. Students will present the activities to the teacher through the ILIAS platform for correction.	10%
Online laboratory practices	Presentation of practice questionnaires in electronic format within the established period Practice exam in synchronous online format	Attendance to all video calls established for online practice sessions as well as the presentation of the practice questionnaires are mandatory and essential to pass the course. The qualification of the practice quiestionnaires will suppose 10% of the global qualification of the subject and the practical exam will represent 30%. The practice exam will consist of two parts corresponding to practice lessons 1-5 and 6-10. A mínimum grade of 4 points is required in each part of the practical exam in order to consider these marks together with those obtained in the seminar clases, practical questionnaires and theory exams to carry out the weighted calculation of the final grade.	40%

If the course is not approved in the Ordinary 1 convocatory, the marks of the complete passed parts will be saved only for the Extraordinary 2. If students don´t pass the final exams, they will have to repeat all the aspects to be evaluated in the subject in the next year.

Extraordinary Convocatory

Assessment probe	Format (classroom/ online synchronous or asynchronous)	Description	Percentage
Assessment of theoretical knowledge	Online synchronous	Online forms made up of test questions and short answer questions using the Google Forms application and/or the UJA ILIAS platform. The theory exam will consist of two parts corresponding to the blocks of lessons 1-4 and 5-8. It will be necessary to obtain a minimum grade of 5 points in each part of the theory exam in order to consider the grade obtained in the rest of activities carried out in the subject.	50%
Assessment of the activities proposed in the online seminar classes	Presentation of activities in electronic format within the established period	The activities carried out in the online seminar classes will be graded. Students will present the activities to the teacher through the ILIAS platform for correction.	10%
Online laboratory practices	Presentation of practice questionnaires in electronic format within the established period Practice exam in synchronous online format	Attendance to all video calls established for online practice sessions as well as the presentation of the practice questionnaires are mandatory and essential to pass the course. The qualification of the practice quiestionnaires will suppose 10% of the global qualification of the subject and the practical exam will represent 30%. The practice exam will consist of two parts corresponding to practice lessons 1-5 and 6-10. A mínimum grade of 4 points is required in each part of the practical exam in order to consider these marks together with those obtained in the seminar clases, practical questionnaires and theory exams to carry out the weighted calculation of the final grade.	40%

RESOURCES

Adaptation of teaching to the multimodal or mixed and totally online model will be carried out using the following resources:

Google Meet to conduct teaching videoconferences and individual and group tutoring, all synchronously.
Google Forms to carry out continuous assesment activities and exams.
ILIAS platform for virtual teaching at the University of Jaén to carry out forms, exams and provide teaching materials to students.
Email from the UJA to send notices to the students and to attend asynchronous individual tutorials.
Bibliographic resources from the Library of the University of Jaén ( https://www.ujaen.es/servicios/biblio ).

Institution in charge of data processing: Universidad de Ja�n, Campus Las Lagunillas, s/n, 23071 Ja�n

Data Protection Delegate: dpo@ujaen.es

Purpose: In accordance with the Universities Law and other national and regional regulations in force, carrying out exams and assessment tests corresponding to the courses students are registered in. In order to avoid frauds while sitting the exam, the exam will be answered using a videoconference system, being able the academic staff of the University of Ja�n to compare and contrast the image of the person who is answering the exam with the student's photographic files. Likewise, in order to provide the exam with evidential content for revisions or claims, in accordance with current regulation frameworks, the exam will be recorded and stored.

Legitimacy: compliance with legal obligations (Universities Law) and other national and regional regulations currently in force.

Addressees: service providers who are the owners of the platforms where the exams are carried out and with whom the University of Ja�n has signed the corresponding data access contracts.

Storage periods: those established in current in force regulations. In the specific case of exam videoconference recordings, not before the examination records and transcripts are closed or the exam can still be reviewed or challenged.

Rights: you can exercise your right of access, amendment, cancellation, opposition, suppression, limitation and portability by sending a letter to the postal or electronic address indicated above. In the event that you consider that your rights have been violated, you may submit a complaint to the Andalusian Council for Transparency and Data Protection www.ctpdandalucia.es

Person in charge: Universidad de Ja�n, Paraje Las Lagunillas, s/n; Tel.953 212121; www.ujaen.es

Data protection delegate (DPO): TELEF�NICA, S.A.U. ; Email: dpo@ujaen.es

Procedure aim: To manage proper recordings of teaching sessions with the aim of facilitating learning process under a multimodal and/or online teaching

Period for record storage: Images will be kept during legal term according to regulations in force

Legitimacy: Data will be managed according to legal regulations (Organic Law 6/2001, December 21, on Universities) and given consent provided by selecting corresponding box in legal admission documents

Data recipients (transfers or assignments): Any person allowed to get access to every teaching modality

Rights: You may exercise your rights of access, rectification, cancellation, portability, limitation of processing, deletion or, where appropriate, opposition. To exercise these rights, you must submit a written request to the Information, Registration and Electronic Administration Service of the University of Jaen at the address above, or by e-mail to the address above. You must specify which of these rights you are requesting to be satisfied and, at the same time, you must attach a photocopy of your ID card or equivalent identification document. In case you act through a representative, legal or voluntary, you must also provide a document that proves this representation and identification. Likewise, if you consider that your right to personal data protection has been violated, you may file a complaint with the Andalusian Data Protection and Transparency Council www.ctpdandalucia.es

DEGREE:	Grado en Biología
FACULTY:	FACULTY OF EXPERIMENTAL SCIENCES
ACADEMIC YEAR:	2020-21
COURSE:	Lab Methods and Instruments

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Syllabus 2020-21 - 10211006 - Lab Methods and Instruments (Métodos e instrumentación de laboratorio)

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