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Syllabus 2019-20 - 76712005 - Protein and Metabolite Purification and Analysis Techniques (Técnicas de purificación y análisis de proteínas y metabolitos)

Caption
  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Máster Univ. en Biotecnología y biomedicina (76712005)
FACULTY: Centre for Postgraduate Studies of the University of Jaen

DEGREE: Doble Máster en Biotecnología y Biomedicina y MFPES (Profesorado) (77912002)
FACULTY: Centre for Postgraduate Studies of the University of Jaen

ACADEMIC YEAR: 2019-20
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Protein and Metabolite Purification and Analysis Techniques
CODE: 76712005 (*) ACADEMIC YEAR: 2019-20
LANGUAGE: English LEVEL: 2
ECTS CREDITS: 6.0 YEAR: 1 SEMESTER: PC
 
2. LECTURER BASIC INFORMATION
NAME: PEDRAJAS CABRERA, JOSÉ RAFAEL
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 302 E-MAIL: pedrajas@ujaen.es P: 953212767
WEBSITE: http://www4.ujaen.es/~pedrajas/
LANGUAGE: - LEVEL: 1
 
NAME: MARTÍNEZ LARA, ESTHER DEL PILAR
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 357 E-MAIL: elara@ujaen.es P: 953212766
WEBSITE: https://www.uja.es/investigacion-y-transferencia/grupos-de-investigacion/estres-celular-y-edad
LANGUAGE: - LEVEL: 1
 
NAME: PERAGÓN SÁNCHEZ, JUAN
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 356 E-MAIL: jperagon@ujaen.es P: 953212523
WEBSITE: www4.ujaen.es/~jperagon/
LANGUAGE: - LEVEL: 2
 
NAME: SILES RIVAS, EVA
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - B3-364 E-MAIL: esiles@ujaen.es P: 953212705
WEBSITE: https://www.uja.es/investigacion-y-transferencia/grupos-de-investigacion/estres-celular-y-edad
LANGUAGE: - LEVEL: 1
 
NAME: VALDERRAMA RODRÍGUEZ, RAQUEL
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 341 E-MAIL: ravalde@ujaen.es P: 953-212768
WEBSITE: ravalde@ujaen.es
LANGUAGE: - LEVEL: 1
 
3. CONTENT DESCRIPTION

This subject offers theoretical and practical training in the following aspects:

  • Structural and functional fundamentals of proteins.
  • Methods of protein analysis and identification
  • Kinetic characterization of enzymes.
  • Preparation of samples for protein purification
  • Protein production by recombinant DNA
  • Chromatographic strategies for the purification of native and recombinant proteins.
  • Applications with recombinant proteins
  • Fundamentals of metabolomics
  • Methods for the analysis of metabolites

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

Theoretical lessons taught by teachers with support of audiovisual resources.

Practical activities in the laboratory. Students should prepare reports of the results.

Performance of exercises by students.

Students may have notes, experimental protocols, bibliographic material, etc. contributed by the teachers through a virtual teaching platform.

 

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

  • Attendance (10%)
  • Active participation (10%)
  • Technical skills and quality of laboratory report (80%)

6. BOOKLIST
MAIN BOOKLIST:
  • The handbook of metabonomics and metabolomics [Recurso electrónico] . Edition: -. Author: -. Publisher: Amsterdam &#59; Oxford : Elsevier, 2007.  (Library)
  • Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation. Edition: -. Author: B. Álvarez Sánchez, F. Priego Capote, M.D. Luque de Castro. Publisher: Trends Anal. Chem. 29 (2010) 120-127  (Library)
  • Metabolomics analysis II. Preparation of biological samples prior to detection. Edition: -. Author: B. Álvarez Sánchez, F. Priego Capote, M.D. Luque de Castro. . Publisher: Trends Anal. Chem. 29 (2010)  (Library)
  • Metabolite identification and quantitation in LC-MS/MS-based metabolomics. Edition: -. Author: J.F. Xiao, B. Zhou, H.W. Ressom. Publisher: Trends Anal. Chem. 32 (2012) 1-14  (Library)
  • Preparation of urine samples prior to targeted or untargeted metabolomics mass-spectrometry analysis. Edition: -. Author: -. Publisher: Trends Anal. Chem. 41 (2012) 75-85  (Library)
  • Basic methods in protein: purification and analysis : a laboratory manual . Edition: -. Author: -. Publisher: Cold Spring Harbor : Cold Spring Harbor Laboratory, 2009  (Library)
  • Protein purification techniques: a practical approach. Edition: 2nd ed. Author: -. Publisher: Oxford: Oxford University Press, 2001  (Library)
  • Protein purification applications: a practical approach. Edition: 2nd ed. Author: -. Publisher: Oxford: Oxford University Press, 2001  (Library)
  • Enzyme assays: a practical approach. Edition: -. Author: -. Publisher: Oxford [etc.]: Oxford University Press Press, 2002  (Library)
  • Molecular and Cellular Enzymology [Recurso electrónico]. Edition: -. Author: Yon-Kahn, Jeannine. Publisher: Berlin, Heidelberg : Springer-Verlag Berlin Heidelberg, 2010.  (Library)
  • Advances in enzymology and related areas of molecular biology. Volume 76 . Edition: -. Author: -. Publisher: New York : John Wiley & Sons, 2009  (Library)
  • Guide to protein purification.. Edition: 2ª ed. Author: -. Publisher: San Diego [etc.] : Academic Press, cop. 2009  (Library)
ADDITIONAL BOOKLIST:
  • Principles and techniques of biochemistry and molecular biology. Edition: 7th ed. Author: -. Publisher: New York : Cambridge University Press, 2013  (Library)