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Syllabus 2019-20 - 10313001 - Chemical Analysis of Biological and Environmental Samples (Análisis químico de muestras biológicas y medioambientales)

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  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Grado en Químicas
FACULTY: FACULTY OF EXPERIMENTAL SCIENCES

ACADEMIC YEAR: 2019-20
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Chemical Analysis of Biological and Environmental Samples
CODE: 10313001 ACADEMIC YEAR: 2019-20
LANGUAGE: English LEVEL: 1
ECTS CREDITS: 6.0 YEAR: 3, 4 SEMESTER: PC
 
2. LECTURER BASIC INFORMATION
NAME: RUIZ MEDINA, ANTONIO
DEPARTMENT: U127 - QUIMÍCA FÍSICA Y ANALÍTICA
FIELD OF STUDY: 750 - QUÍMICA ANALÍTICA
OFFICE NO.: B3 - 124 E-MAIL: anruiz@ujaen.es P: 953-212759
WEBSITE: http://www4.ujaen.es/~anruiz/
LANGUAGE: English LEVEL: 1
 
3. CONTENT DESCRIPTION

THEORY DEVELOPED

SECTION I: INTRODUCTION TO THE CLINICAL AND ENVIRONMENTAL ANALYTICAL CHEMISTRY. ANALYSIS TECHNIQUES

Item 1. BASIC PRINCIPLES OF CLINICAL LABORATORY AND ENVIRONMENTAL ANALYSIS. Measuring units. Mass measurements. La boratory equipment. Laboratory water. Security in the clinical laboratory.


Item 2. ANALYTICAL TECHNIQUES FREQUENTLY USED  (I). Molecular absorption spectrometry. Fluorimetry. Turbidimetry and nephelometry. Luminometry. Atomic spectrometry. Osmometry. Chromatography. Electrophoresis. Electrochemical techniques.


Item 3. ANALYTICAL TECHNIQUES FREQUENTLY USED (II). Immunochemical techniques. Production of antiserum and monoclonal antibodies. Agglutination reactions and immunoprecipitation. Immunoassay with labeled reagents. Automation.

 
SECTION II:  CLINICAL  ANALYTICAL CHEMISTRY 

Item 4. ANALYSIS OF BLOOD. Sample types. Collection containers. Variables Collection. Laboratory criteria for unacceptable specimens. Sample processing. Errors in the clinical laboratory. Quality control program.

Item 5. ANALYSIS OF URINE. Renal physiology. Urinalysis. Physical examination of the urine. Chemical examination. Microscopic examination

Item 6. METHODS OF ANALYSIS OF AMINO ACIDS, PROTEINS, CARBOHYDRATES, LIPIDS, VITAMINS AND ENZYMES. Definition and classification. Chemical, physical and biological properties. Specific analysis of clinical parameters: total serum protein, Urea, Albumin, Glucose, Insulin, Amniotic Fluid Phospholipids, Cholesterol, Triglycerides, and Vitamins (water soluble and fat soluble).

 
SECTION III: ENVIRONMENTAL ANALYTICAL CHEMISTRY

Item 7. ENVIRONMENTAL ANALYTICAL CHEMISTRY AND ITS SCOPE. Concept of contamination. Classification of chemical contaminants. Transport of pollutants. Criteria for the selection of the analysis method.


Item 8. ATMOSPHERIC ANALYSIS: GASES AND PARTICLES. Composition of the atmosphere, air pollutants and their common sources. Sampling and preconcentration. Analysis of gases. Sources of suspended solids. Measures of total suspended matter. Techniques for particle size discrimination. Particle analysis. Remote sensing techniques.

Item 9. WATER ANALYSIS: MAJOR  CONSTITUENTS AND TRACE CONTAMINANTS. Water quality: natural and wastewater. Sampling. Analysis of general parameters. Determination of gases and non-specific organic compounds. Analysis of major anionic and cationic components. Trace metal ions. Organic contaminants at trace levels.


Item 10. ANALYSIS OF SOLID SAMPLES. Types of solid samples. Sampling and storage. Determination of organic compounds. Analysis m ethods.

 

PRACTICAL PROGRAM DEVELOPED

Session 1

Practice 1: Determination of iron in serum.

Practice 2: Determination of albumin in serum.

Session 2

Practice 3: Determination of bicarbonate in serum.

Practice 4: Determination of ascorbic acid in urine by the official method of analysis (AOAC).

Session 3

Practice 5: Enzymatic determination of glucose in serum.

Practice 6: Determination of turbidity in water.

Session 4

Practice 7: Determination of dissolved oxygen in water.

Lesson 8: Determination of total organic matter in a soil sample.

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

1.- The lectures will be taught primarily as exposing lectures.

 
2.-  The student will attend a mandatory practice sessions that will be conducted the analysis of chemical compounds in different biological and environmental matrices.  

3. The students will develop Academically Supervised Activities: (a)  Seminars / Presentations: each student will select a topic of clinical or environmental interest which will be finally exposed in class to other colleagues; (b) E xercises, Problems and Test.

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

The theoretical examinationshall be written andwill be heldon the date setby the Faculty ofExperimental Sciences.

The practical sessions will be mandatory. The knowledge gained will be evaluated from the lab notebook.

To overcome the Academically Supervised Activities will be taken into account attendance, which is mandatory, and the work to be developed.

6. BOOKLIST
MAIN BOOKLIST:
  • Clinical chemistry: concepts and applications. Edition: -. Author: Anderson, Shauna C.. Publisher: New York [etc.]: McGraw-Hill, 2007  (Library)
  • Introduction to environmental analysis. Edition: -. Author: Reeve, Roger N.. Publisher: Chichester [etc.]: John Wiley, 2002  (Library)
  • Environmental chemistry. Edition: 9th ed.. Author: Manahan, Stanley E.. Publisher: Boca Raton (Florida) : CRC, cop. 2009.  (Library)
  • Environmental analytical chemistry. Edition: 2nd. ed.. Author: -. Publisher: London [etc.] : Blackie Academic & Professional, cop. 2000.  (Library)
ADDITIONAL BOOKLIST:
  • Tietz textbook of clinical chemistry and molecular diagnostics. Edition: 4th ed. Author: -. Publisher: St. Louis : Elsevier-Saunders, cop. 2006  (Library)
  • Environmental analysis: techniques, applications and quality assurance. Edition: -. Author: -. Publisher: Amsterdam [etc.]: Elsevier, 1993  (Library)