Universidad de Jaén

THEORETICAL CONTENTS

Topic 1. Biological bases of Human Reproduction. Endocrine system. Male reproductive system. Female reproductive system.

Topic 2. Gametogenesis Spermatogenesis Oogenesis Ovulation. Endocrine control of ovulation

Topic 3. Fertilization. Embryonic development. Sperm and ovule transport. Sperm training and activation. Phases of embryonic development. Gastrulation Neurulation Organogenesis

Topic 4 . Bases of Sterility. Identification of the causes of infertility. Female infertility. Male infertility Special categories of infertility: genetic infertility and immunological infertility. Sterility diagnosis. Female sterility treatment

Topic 5 . Analysis of semen. Seminal analysis and sperm tests. Bank of semen

Topic 6. Assisted Reproduction Techniques: artificial insemination, "in vitro" fertilization, sperm microinjection. MACS transfer. IMSI. "Hatching" assisted

Topic 7. In Vitro Production of Embryos. Observation and selection of embryos. Embryo culture Co-culture techniques. Freezing and storage of embryos. Defreezing of embryos

Topic 8 . Multiplication of Embryos and Gametes. Reproductive process Handling. Cloning Nuclear transfer. Cloning techniques. Applications of cloning. Ethical considerations of cloning

Topic 9. Determination of Sex Gonadal characteristics. Phenotypic characteristics. Molecular determination of sex. Sex determination techniques

Topic 10 . Cryobiology Preservation of paternal and maternal inheritance - Preservation of embryos. Microscopic evaluation Vitrification

Topic 11. Genetic diagnosis Pre-implantation diagnosis "Screening" aneuploidy

Topic 12. Biological Safety and Assisted Reproduction Laboratory. General basics of biological safety. Biological safety in cryopreservation. Transport of reproductive biological material. Action protocols. Evaluation of biological risks

Topic 13. Legal and ethical aspects of Assisted Human Reproduction. Spanish and European legislation. Ethical aspects in research and experimentation

Topic 14 . Basic and applied research in Human Reproduction

PRACTICAL CONTENTS

Practice 1. Realization of Seminogram. Comparison of fresh and cryopreserved semen samples with different diluents

Practice 2. Study of the variations of sperm motility at different pHs. In vitro study

Practice 3. Effect of the increase of the temperature on the spermatozoa. In vitro study

Practice 4 and 5. Determine the expression of androgen receptors in male reproductive by immunohistochistry

Practice 6 and 7 . Determination of the Estrogen Receptor in female reproductive tract by Immunohistochemistry

Practice 8 . Histochemical Study of Female and Male Reproductive

Practice 9. Quantification of estrogen expression in ovarian, horn and uterus and androgen receptors in testicle

Practice 10 . Estral cycle of the female mouse

Magistral classes in a large group. Practical laboratory classes in small group. Seminars, exposition of works and debates with problem solving

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

For the evaluation of the theoretical knowledge of the subject a theoretical written probe will be carried out in the official date. The subject of this exam will be eliminated with a grade equal to or higher than 5 out of 10. The mark of the theoretical part represents 60% of the final grade. Through the written exam test we will also evaluate the Skills CT6, CT7, CT8, CT9, CT10 and CG11     

For the evaluation of laboratory practical knowledge, the completion of practices is a mandatory condition. The students must make and present a global notebook of practices in which they respond to the questions that are presented to them and present the results obtained in the practice sessions that will be evaluated and will correspond to 20% of the grade. Those students who do not do the practices will not have the 20% corresponding to practices in the computation of the final grade. In the realization of the sessions of practices will evaluate the Skills CE4, CT2, CT5, CT6, CT7 and CG8 The formative activities will be evaluated by the professor where the realization of the same will be taken into account, as well as the exposition of the works. It assumes 10% of the final grade. Students who do not perform these activities on an ongoing basis may take an oral exam consisting of an exhibition-debate of a topic related to the theoretical or practical contents of the subject. With the accomplishment of the formative activities we will evaluate the skills CE4, CT1, CT2, CT3 and CT4. The attendance to practices and to the formative activities will be evaluated and will suppose 10% of the final note   

It is possible to pass the subject through continuous assessment, without the need for a final theoretical examination, provided that all the academic activities are carried out: theoretical, practical and seminars and are passed with a grade equal to or greater than 5 out of 10.