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Syllabus 2019-20 - 10213004 - Metabolic and Molecular Biochemistry (Bioquímica metabólica y molecular)

Caption
  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Grado en Biología
FACULTY: FACULTY OF EXPERIMENTAL SCIENCES

ACADEMIC YEAR: 2019-20
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Metabolic and Molecular Biochemistry
CODE: 10213004 ACADEMIC YEAR: 2019-20
LANGUAGE: English LEVEL: 2
ECTS CREDITS: 6.0 YEAR: 4 SEMESTER: PC
 
2. LECTURER BASIC INFORMATION
NAME: SILES RIVAS, EVA
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - B3-364 E-MAIL: esiles@ujaen.es P: 953212705
WEBSITE: https://www.uja.es/investigacion-y-transferencia/grupos-de-investigacion/estres-celular-y-edad
LANGUAGE: English LEVEL: 2
 
NAME: PERAGÓN SÁNCHEZ, JUAN
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 356 E-MAIL: jperagon@ujaen.es P: 953212523
WEBSITE: www4.ujaen.es/~jperagon/
LANGUAGE: English LEVEL: 2
 
3. CONTENT DESCRIPTION

Theoric curriculum

  1. Overview of regulation and integration of metabolism.
  2. Signal transduction systems. cAMP-dependent pathway, inositol polyphosphate-dependent pathway, receptor tyrosine kinases (the insulin receptor), cGMP and nitric oxide-dependent pathways, and steroid hormone receptors.
  3. Metabolism of carbohydrates, lipids and nitrogenous compounds and its regulation. Glycolisis and gluconeogenesis. Metabolism of glycogen. Pentose phosphate pathway. Digestion, absorption and transport of lipids. Biosynthesis and catabolism of fatty acids. Ketone bodies. Biosynthesis of triacylglycerols and  membrane lipids. Metabolism of nucleotides. General aspects of amino acid metabolism. The urea cycle.
  4. Regulation of gene expression. Regulation of gene expression in prokaryotes: Lactose operon. Arabinose operon. Tryptophan operon. Lysogenic and lytic growth in lambda phage. Small ARNs. Regulation of gene expression in eukaryotes: Chromatin structure and gene expression. Transcription factors and its interaction with DNA. Post-transcriptional regulation.
  5. Protein folding, maturation, sorting and degradation. Mechanisms of protein folding. Post-translational modifications of proteins. Protein-sorting pathways. Protein degradation: The ubiquitin-proteasome pathway and lysosomal proteolysis.
  6. Methods for the study of  gene and protein expression.  Western-blot. Proteomics. Northern-blot. PCR. Real-time PCR. DNA arrays.  

Laboratory practice

Effect of starvation on rat-liver glycogen content and glucose 6-phosphate dehydrogenase activity and expression.

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

TEACHING METHODOLOGY:

a) Academic sessions to the great group of students: 26 hours (2,6 ECTS)

b) Academic sessions to the small group of students: 32 hours (3,2 ECTS)

c) Collective tutorial sessions: 2 hours (0,2 ECTS)

TEACHING METHODOLOGY FOR PATIE STUDENTS:

a)  Lecture (Theory). Tutorial sessions, proposal of activities and exercises, and supply of printted material in English (in presence of professor or virtual), 1 hour per week

b) Lecture (Practice): Explanation of laboratory practice in English, 20 hours

c) Seminars: 0-2 hours per week

c) Homework: 4 hours per week.

 

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

Assessment methodology:

1) Exam of theoric curriculum (65%); 2) compliance with the practices (25%) and 3) seminars and other activities (10%).

6. BOOKLIST
MAIN BOOKLIST:
  • LEHNINGER PRINCIPLES OF BIOCHEMISTRY: INTERNATIONAL EDITION. Edition: 7ª Edición. Author: Nelson, Cox. Publisher: W.H.FREEMAN & CO LTD
  • Current protocols essential laboratory techniques. Edition: -. Author: -. Publisher: Hoboken, N.J. : John Wiley & Sons, c2008  (Library)
  • Textbook of biochemistry : with clinical correlations. Edition: 7 th ed. Author: -. Publisher: Hoboken, N.J : John Wiley &Sons, Inc. , 2011  (Library)
ADDITIONAL BOOKLIST:
  • Molecular cell biology. Edition: 6th ed., 1st print.. Author: -. Publisher: New York : W. H. Freeman and Co., cop. 2008  (Library)
  • Harper's illustrated biochemistry. Edition: 27th ed. Author: -. Publisher: New York (etc.): McGraw-Hill, 2006  (Library)
  • Principles of biochemistry. Edition: 5th ed. Author: -. Publisher: Boston : Pearson, c2014  (Library)
  • Biochemistry. The Chemical Reactions of Living Cells.. Edition: 2ª. Author: Metzler. Publisher: Harcourt Academic Press
  • Molecular Biology of the cell. Edition: 6ª. Author: Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter. Publisher: Garland Science: New York and Abingdon