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Syllabus 2019-20 - 10213001 - Genetic Engineering, Transgenesis and Improvement (Ingeniería genética, transgénesis y mejora)

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  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Grado en Biología
FACULTY: FACULTY OF EXPERIMENTAL SCIENCES

ACADEMIC YEAR: 2019-20
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Genetic Engineering, Transgenesis and Improvement
CODE: 10213001 ACADEMIC YEAR: 2019-20
LANGUAGE: English LEVEL: 1
ECTS CREDITS: 6.0 YEAR: 4 SEMESTER: PC
 
2. LECTURER BASIC INFORMATION
NAME: BULLEJOS MARTÍN, MÓNICA
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 420 - GENÉTICA
OFFICE NO.: B3 - 358 E-MAIL: bullejos@ujaen.es P: 953212770
WEBSITE: http://www.ujaen.es/investiga/cvi220/index.htm
LANGUAGE: English LEVEL: 1
 
3. CONTENT DESCRIPTION

THEORY

PART I: Manipulation of nucleic acids in vitro. Basic techniques.

1. Introduction to genetic engineering. Basic techniques to manipulate nucleic acids in vitro. Historical perspective of the technology of analysis and manipulation of nucleic acids. Purification of DNA and RNA. Separation and analysis of nucleic acids by gel electrophoresis.

2. Enzymes used to manipulate nucleic acids in vitro. Cut and joining of DNA. Chemical modification in vitro. Synthesis and degradation of NNAA.

3. Amplification of NNAA in vitro (PCR) and applications. Polymerase Chain Reaction (PCR): Taq polymerase, primers and amplification. PCR using RNA (RT-PCR). Applications of the PCR.

4. Nucleic acid hybridization applied to sequence identification. Transfer of NNAA to membranes. Probes. Hybridization. Detection.

5. Gene expression analysis. Northern-blot, RNase protection assays, RT-PCR, in situ hybridization, primer extension assay.

PART II: Genetic manipulation in prokaryotes ( E. coli)

6. Cloning and expression in prokaryotes ( E. coli), Part I: Introduction to cloning. Cloning vectors commonly used in prokaryotes. Methods to introduce new genetic information in prokaryotes.

7. Cloning and expression in prokaryotes ( E. coli), Part II: Detection and identification of positive clones: selection of recombinants.

8. Cloning and expression in prokaryotes ( E. coli), Part III: Cloning and expression of recombinant DNA strategies in E. coli: Libraries.

9. Sequence manipulation: in vitro mutagenesis. Random and directed mutagenesis.

PART III: Genetic manipulation in eukaryotes

10. Genetic manipulation and genetic improvement in microorganisms. Applications. Yeast as eukaryotic model organism. Biotechnological importance of yeast. Genetic engeniering in yeast and other fungi. Examples and applications.

11. Genetic manipulation and genetic improvement in plant. Applications. Classical plant improvement. Genetic manipulation techniques in plants. Transgenic plants. Examples and applications.

12. Genetic manipulation and genetic improvement in animals. Applications. Classical animal improvement. Genetic modification of cells and complete organisms. Growth and production. Some applications.

13. Legislation about the use of genetic modified organisms and patents. Economical, ethical and cultural aspects. Legislation.

 

PRACTICAL LESSONS

A) Laboratory

Laboratory practical lessons will be taught in 2 weeks (2 hour session per day). The procedures performed include several techniques on NNAA manipulation reviewed in theory.

PRACTICE 1 . Southern-Blot. This practical includes learning of nucleic acids labelling techniques, labelling quantification, nucleic acid transfer to nylon membranes, in situ hybridization with a labelled probe and detecting the label by immunological reactions. The objective is to identify DNA specific sequences in genomic or plasmidic samples.

PRÁCTICA 2 . Cloning in E. coli. This practical lesson includes: DNA restriction and ligation (synthesis of recombinant DNA), obtaining bacterial competent cells, transformation of competent cells with recombinant DNA (previously obtained in class) selection of recombinant bacteria, extraction of plasmid DNA, restriction and electroforesis of plasmid DNA and screening for the presence of insert.

B) Formative Activities

Applications of PCR

Alternatives to DNA ligases to obtain recombinant DNA

NGS platforms.

Applications of recombinant proteins.

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

Lectures: Big group - Lectures where a global and integrated view of the units of several blocks are discussed. At the end of thematic blocks, short questions about the subjects included in the lessons will be discussed and solved with students.

Formative activities: Small groups - Problem solving lectures where knoledge provided in theoretical lectures will be applied. Using real data, students have to apply their knoledge and the scientific method to explain results obtained.

Practical lessons: Laboratory lessons in small groups - Stidents will perform experiments in the laboratory related with concepts explained in theory. In these lessons the students will have a detailes protocol that will have to follow to fulfill the objectives proposed.

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

Call "Ordinaria I":

Continuous assessment considering the following levels:

- Written exam and level assessment (in English) (70 % = 60% exam + 10% level assessment) to evaluate the knowledge obtained by the student during the course, specially the ability to apply it to real situations. To pass the subject, qualifications bigger than 4 (out of 10) in the exam will be required.

- Practical lessons (15 %): It will be evaluated attendance to practical lessons, understanding and results obtained.

- Formative Activities (15 %): It will be evaluated student participation, the quality of student participation, problem solving capabilities and completion of proposed activities.

Call "Extraordinaria II":

- Final exam (100%).

6. BOOKLIST
MAIN BOOKLIST:
  • Gene cloning: an introduction. Edition: 3rd ed., [repr.]. Author: Brown, T. A.. Publisher: London [etc.]: Chapman, 1996.
    • Notes: Edición 2006 no disponible en biblioteca
     (Library)
  • Genomics : applications in human biology. Edition: -. Author: Primrose, S.B.. Publisher: Malden: Blackwell, cop. 2004.
    • Notes: Existe edición 2006 no disponible en biblioteca
     (Library)
  • Molecular cloning : a laboratory manual. Edition: 4th ed. Author: Green, Michael R. (Michael Richard), 1954-. Publisher: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, 2012  (Library)
  • Analysis of genes and genomes. Edition: -. Author: Reece, Richard J.. Publisher: Chichester : John Wiley & Sons, cop. 2004.  (Library)
  • Recombinant DNA: genes and genomes : a short course. Edition: 3rd ed.. Author: -. Publisher: New York : W.H. Freeman : Cold Spring Harbor Laboratory, 2007.  (Library)
  • Understanding DNA and gene clonig: a guide for the curious. Edition: 4th. ed. Author: Drlica, Karl. Publisher: New York: John Wiley & Sons, cop. 2004  (Library)
  • From genes to genomes: concepts and applications of DNA technology. Edition: Repr. with corr. Author: Dale, Jeremy W.. Publisher: Chichester: John Wiley & Sons, 2003.
    • Notes: Existe una edición de 2007 no disponible en biblioteca
     (Library)
  • Genetics: from genes to genomes. Edition: 4th ed.. Author: -. Publisher: Boston [etc.] : McGraw-Hill, 2011  (Library)
  • Biotechnology. Edition: 2. Author: David P. Clark, Nanette J. Pazdernik. Publisher: Elsevier
ADDITIONAL BOOKLIST:
  • Cloning, gene expression and protein purification: experimental procedures and process rationale. Edition: -. Author: -. Publisher: Oxford : Oxford University Press , 2001.  (Library)
  • Molecular analysis and genome discovery. Edition: -. Author: -. Publisher: Chichester: John Wiley & Sons, cop. 2004  (Library)
  • Genes, girls, and gamow: after the double helix. Edition: -. Author: Watson, James D.. Publisher: New York: Vintage Books, cop. 2003  (Library)
  • DNA: the secret of life. Edition: -. Author: Watson, James D.. Publisher: New York : Alfred A. Knopf, 2003  (Library)
  • Lateral DNA transfer: mechanisms and consequences. Edition: -. Author: Bushman, Frederic. Publisher: Cold Spring Harbor : Cold Spring Harbor Laboratory Press, cop. 2002.  (Library)
  • Transgenic plants: current innovations and future trends. Edition: -. Author: -. Publisher: Wymondham: Horizon Scientific Press, 2003  (Library)
  • The cloning sourcebook [Recurso electrónico]. Edition: Rev. pbk. ed.. Author: -. Publisher: New York ; Oxford : Oxford University Press, 2003.  (Library)
  • Molecular biotechnology [Recurso electrónico] : principles and applications of recombinant DNA. Edition: 4th ed.. Author: Glick, Bernard R.. Publisher: Washington, DC : ASM Press, c2010.  (Library)