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Syllabus 2018-19 - 10312018 - Biochemistry (Bioquímica)

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  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Grado en Químicas
FACULTY: FACULTY OF EXPERIMENTAL SCIENCES

ACADEMIC YEAR: 2018-19
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Biochemistry
CODE: 10312018 ACADEMIC YEAR: 2018-19
LANGUAGE: English LEVEL: 1
ECTS CREDITS: 9.0 YEAR: 4 SEMESTER: SC
 
2. LECTURER BASIC INFORMATION
NAME: PEDRAJAS CABRERA, JOSÉ RAFAEL
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 302 E-MAIL: pedrajas@ujaen.es P: 953212767
WEBSITE: http://www4.ujaen.es/~pedrajas/
LANGUAGE: - LEVEL: 1
 
NAME: CAÑUELO NAVARRO, ANA ROSA
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 302 E-MAIL: acanuelo@ujaen.es P: 2767
WEBSITE: http://www4.ujaen.es/~acanuelo/
LANGUAGE: - LEVEL: 1
 
3. CONTENT DESCRIPTION

PROGRAM OF THEORY

1: Introduction to Biochemistry and Molecular Biology.

2: Amino acids. The peptide bond. Protein structures.

3: Structure-function relationships of proteins.

4: Proteomic.

5: Enzymes: Classification. Catalytic mechanisms. Coenzymes. Kinetic parameters. Inhibitors of enzymes. Allosterism in enzymes. Isoenzymes. Zymogens.

6: Nucleotides. Nucleic acids: DNA and RNA. Structure of nucleic acids.

7: DNA replication. Mutations. Repair mechanisms.

8: Transcription: biological synthesis of RNA

9: Translation: biological synthesis of proteins.

10: Technical handling of nucleic acids. Purification. Hybridization. The polymerase chain reaction. Recombinant DNA technology. Genomics.

11: Thermodynamics of biochemical processes. Gibbs free energy. Energy of biological redox reactions.

12: Metabolism: Metabolic pathways. Catabolism and anabolism. General mechanisms of metabolic regulation.

13: The Krebs cycle. Anaplerotic reactions. Ketones.

14: Oxidative phosphorylation. Photophosphorylation.

15: Carbohydrates.

16: Metabolism of carbohydrates: Glycolysis. Fermentations. Glycogen synthesis and degradation. Gluconeogenesis. Pentose phosphate pathway. The Calvin-Beson cycle.

17: Lipids.

18: Lipid Metabolism: Degradation and synthesis of fatty acid. Synthesis of triacylglycerols and glycerophospholipids. Cholesterol synthesis. Lipid mobilization in human. Lipoproteins.

19: Metabolism of nitrogenous compounds: Degradation of proteins and amino acids. Deamination and transamination. The urea cycle. Nucleotide synthesis and degradation.

20: Metabolic profile of the main human organs and hormonal regulation.

 

PROGRAM OF LABORATORY

Session 1: Purification of a protein by affinity chromatography.

Session 2: Protein electrophoresis (SDS-PAGE)

Session 3: Western-blot

Session 4: Kinetic characterization of an enzyme activity.

Session 5: Analysis of glycogen.

Session 6: Analysis of triacylglycerols and cholesterol in blood samples. Analysis of lipid peroxidation derivatives in foods.

Session 7: Polymerase chain reaction and DNA electrophoresis in agarose gel.

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

Theory classes.

Practical laboratory sessions in small groups where the student will apply different techniques and experimental protocols related to the theoretical contents. The student will have to collect and discuss the obtained results after each practical session.

Practical activities and problem resolution in small groups.

Individual and collective tutorial sessions, where the student will be able to ask and solve questions guided by the professor.

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

Theory will be evaluated by a written test. It is needed to obtain at least an score of 5 of 10 points in this examination in order to add the scores of the other activities to the global qualification.

Attendance to all of the practical sessions will be mandatory (exceptions will be made in case of properly justified situations). Global qualification of practical contents will depend on results obtained in laboratory sessions (50%) and a practices examination (50%).

Attendance to all of the problem resolution sessions will be mandatory (exceptions will be made in case of properly justified situations). Problem resolution sessions will be evaluated accordingly to the results obtained in these sessions.

6. BOOKLIST
MAIN BOOKLIST:
  • Lehninger : principles of biochemistry. Edition: 6th ed. Author: Nelson, David L., 1942-. Publisher: New York : W. H. Freeman and Co, 2013  (Library)