Universidad de Jaén

THEORY

Lesson 1. Introduction

Introduction to trace analysis. Separation techniques in trace analysis. Classification of separation techniques

Lesson 2. Introduction and theory of chromatography. 1.Definitions and classification of chromatographic techniques. Fundamentals and key parameters in chromatography. N, H, resolution, efficiency,… Effect of mobile phase flow rate. Van Deemter equation. Applications. Qualitative and quantitative analysis

Lesson 3. Gas chromatography. 1. Introduction. Classification of GC methods. Instrumentation. Gas. Column. Inyection system. Stationary phases. Temperature in GC. Detectors. GC-GC and GCxGC. Applications.

Lesson 4. Liquid chromatography. 1. Introduction. Instrumentation in HPLC. Pumps. Injection system. Columns. Stationary phases. Chiral chromatography. Mobile phases. Detectors in HPLC. Working modes in HPLC. Effect of temperatura in HPLC. Ultra-high pressure liquid chromatography (UHPLC). LCxLC. Microflow and nanoflow LC. Applications.

Lesson 5. Ion chromatography. Fundamentals of ion chromatography. Stationary phases. Mobile phases. Detection. Ion Suppressor.

Lesson 6. Size exclusion, planar and supercritical fluid chromatography. Size exclusion chromatography. Thin-layer chromatography. Supercritical fluid chromatography

Lesson 7. Capillary Electrophoresis and capillary electrochromatography. 1. Introduction. Basics of capillary electrophoresis. Migration phenomena. Electrophoretic mobility and electroosmotic flow. Instrumentation in capillary electrophoresis. Sample introduction. Detecton. CE modes. Capillary Zone electrophoresis. Micellar electrokinetic chromatography. Isotacophoresis. Isoelectric focusing. Capillary gel electrophoresis. Capillary electrochromatography. Analytical applications.

Lesson 8. Sample treatment for trace analysis. Introduction to sample treatment. Liquid-liquid extraction. Solid-phase extraction (SPE). Solid-phase microextraction (SPME). Stir bar sorptive extraction (SBSE). Headspace (HS). Matrix solid-phase dispersión (MSPD). Soxhlet extraction. Pressurized liquid extraction. Supercritical fluid extraction

Lesson 9. Introduction to mass spectrometry. 1- Instrumentation. Ionization in MS. Electron Impact. Chemical Ionization. Electrospray. APCI. MALDI. Analyzers. Tandem mass spectrometry. Fragmentaiton. High resolution mass spectrometry. Basic of mass spectra interpretation

Lesson 10. Hyphenated techniques I: GC-MS. 1. Instrumentation.2. Applications

Lesson 11. Hyphenated techniques II: LC-MS. 1. Instrumentation.2. Applications

Lesson 12: Elemental mass spectrometry and speciation. ICP-MS: Fundamentals and applications. Hyphenation of ICP-MS and chromatography. Speciation analysis and its applications

Lesson 13. Automation. Introduction. Flow methods. Flow inyection analysis. Sequential injection analysis. Applications

LABORATORY SESSIONS

1. Selected questions and use of HPLC simulator
2. Analytical applications of HPLC
3. Analytical applications of GC

Theoretical classes (38 hours). Lecture notes and material provided in platform or upon request to Professor.

Laboratory sessions (10 hours). A lab report template (hand-out) will be provided.

Seminars (10 hours). 5 sessions (2h) with different practical tasks to accomplish associated to the theoretical contents.

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

Theoretical exam (70 %).

Laboratory session and preparation of lab report (15 %)

Seminars and tasks: presentation of a topic agreed with tutor (15 %)