Universidad de Jaén

1. Introduction to the animal experimentation science. 
   2. Animal welfare and security when working with experimentation animals. 
   3. Management of experimentation animals in captivity. 
   4. Experimental procedures. 
   5. Alternative methodologies to the use of experimentation animals. Celular cultures.

THEORY

Lesson 1. The science of the laboratory animal and ethical principles of the animal experimentation.

Lesson 2. Social perception of research with animals. Laws of animal experimentation.

Lesson 3. General biology and basic requierements for maintaining experimentation animals.

Lesson 4. Microbiological and genetic standardization.

Lesson 5. Animal welfare and prevention of animal pathologies.

Lesson 6. Biosecurity. Prevention, security and hygiene in animal experimentation. Zoonosis.

Lesson 7. Determinant variables in experimentation: physiological, hematological, biochemical and other parameters.

Lesson 8. Animal models.

Lesson 9. Itroduction to experimental procedures.

Lesson 10. Alternative techniques to the animal experimentation. In vitro models and celular cultures: future substitutes of the experimentation animals?

SEMINARS

Environmental enrichment.

Analgesia and anesthesia of experimentation animals. The euthanasia.

Industrial production of fodder. Special diets for experimentation animals.

Debate on the alternative methodologies for the animal experimentation and social impact I.

Debate on the alternative methodologies for the animal experimentation and social impact II.

Bioethics committees, requirements and protocols for authorize experimentation with animals.

Experimentation and scientific research on wild animals: demands and limitations.

Non-invasive techniques of molecular images apllied to animal models.

PRACTICAL LESSONS

1. Equipments and infrastructures.
2. Management, capture and marking of experimentation animals.
3. Methods for obtaining blood samples and/or administration of treatments.
4. Bioassays for detecting marine toxines in molluscs and alternative methods.
5. Characterization of the stress.
6. Determination of the severity index of the experimental procedures. Correcting measures
7. Chemical immobilization of mammals.
8. Diagnosis of echinococcosis or hydatid cyst.
9. Diagnosis of sarcoptic mange. Differential count of mites.
10. Adequate management of laboratoru animals (video). Administration routes for drugs and treatments.
11. Oestral cycle.
12. Metabolic study.
13. Disection.
14. Determination of proteinuria level.
15. Determination of the creatinine level in rat orine.
16. Statistical analyses.
17. Homogenization of tissues.
18. Use of informatic databases for searching alternative methods in animal experimentation.

FORMATIVE ACTIVITIES :

AF1 . Bibliographic search.

AF2 . Didactic videos on ethical issues regarding the use of experimentation animals.

1.- Activities A1 . Expositive lessons will be give to great group, when the theoric contents will be exposed and explained, with a duration of   1   presential hour in classrooms.

2.- Activities A2 .

1. a) The practical lessons and the formative activities (in small groups) will be give in the laboratory. These include M9 and M14-type activities, and M10 activities as well, in the computer room. The duration of each of these activities will be  2 hours .

1. b) Seminars for small groups including M7 and M8-type activities, with a duration of 1 At this point we’ll inititate the use of learning methods based on resolving problems (ABP).

3.- Activity A3 . Tutorial activities of the professors, carried out at their office, for individual students or reduced groups..

4.- Activity A6 . Presential test in the classroom fro great groups for evaluating both the theoretical and practical contents and other competences as well.

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

The 70 % of the overall note will be obtained by the written test, which will evaluate the acquisition of basic knowledge of the matter and the achieve of competences CT1-7, as well as CE-2, CE24, CG3, CT10. The knowledge adquired through the theoretical and practical lessons, formative activities and seminars will be evaluated.

The remaining 30 % of the note will be obtained after evaluating the work of the students in the laboratory, formative activities and seminars. The asistence and participation will be evaluated, as well as the achieve of objectives and competences (CT1-10, CG3-5, CG-7, CG8-9, CE24, CE56, CE63) like a continuous evaluation.