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Syllabus 2015-16 - 72512002 - Proteomics and Dynamics of Proteins (Proteómica y dinámica de proteínas)

Caption
  • Level 1: Tutorial support sessions, materials and exams in this language
  • Level 2: Tutorial support sessions, materials, exams and seminars in this language
  • Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
DEGREE: Máster en Biotecnología y biomedicina
FACULTY: Centro de Estudios de Postgrado
ACADEMIC YEAR: 2015-16
COURSE: Proteomics and Dynamics of Proteins
SYLLABUS
1. COURSE BASIC INFORMATION
NAME: Proteomics and Dynamics of Proteins
CODE: 72512002 ACADEMIC YEAR: 2015-16
LANGUAGE: English LEVEL: 2
ECTS CREDITS: 4.0 YEAR: 1 SEMESTER: AN
2. LECTURER BASIC INFORMATION
NAME: PERAGÓN SÁNCHEZ, JUAN
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 356 E-MAIL: jperagon@ujaen.es P: 953212523
WEBSITE: www4.ujaen.es/~jperagon/
ORCID: https://orcid.org/0000-0002-7502-1119
LANGUAGE: English LEVEL: 2
NAME: MARTÍNEZ LARA, ESTHER DEL PILAR
DEPARTMENT: U103 - BIOLOGÍA EXPERIMENTAL
FIELD OF STUDY: 060 - BIOQUÍMICA Y BIOLOGÍA MOLECULAR
OFFICE NO.: B3 - 357 E-MAIL: elara@ujaen.es P: 953212766
WEBSITE: https://www.uja.es/investigacion-y-transferencia/grupos-de-investigacion/estres-celular-y-edad
ORCID: https://orcid.org/0000-0002-4962-3634
LANGUAGE: English LEVEL: 1
3. CONTENT DESCRIPTION

Theory:

  1. Introduction. Protein characterization and purification studies.
  2. Protein folding.
  3. Protein missfolding and disease.
  4. Protein turnover and cell growth.
  5. Cell protein degradation systems.
  6. Posttranslational modification of proteins.
  7. Protein sorting.
  8. Protein-ligand interactions.
  9. Introduction to proteomics: Fundamentals, techniques, equipments, and applications.

Laboratory Practice:

  1. Mass spectrometry equipment in the University of Jaén.
  2. Equipment and techniques for protein characterization in the Department of Experimental Biology of the University of Jaén.
  3. Introduction to ProteoRed, the Spanish Network for Proteomics.
  4. Effects of starvation on rat hepatic proteome by 2D SDS-PAGE. Sample preparation. 2-DE.Staining and analysis of proteins. Introduction to PDQest R (BioRad Laboratories). MS analysis. Identification of proteins and functional proteomics.

Other activities:

1) Use of database of scientific literature, 2) Standards for Ph.D degree, 3) Structure and preparation of a research paper, 4) Preparation and exposition of one oral presentation.

4. COURSE DESCRIPTION AND TEACHING METHODOLOGY

General methodology:

1) Academic sessions (20 h in presence of professor),

2) Laboratory sessions (15 h)

3) Oral presentations (2.5 h)

  Course description and teaching methodology for PATIE student:

1) Lecture (Theory): Tutorial session, supply of press material, proposal of activities and exercises in English (in presence of professor or virtual): 1 h per week

2) Lecture (Practice): Tutorial session, supply of press material (in presence of professor or virtual): 1 h per week

c) Seminars: Supply of press material to prepare oral presentation: 1 h per week

d) Homework: 4 h per week

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

5. ASSESSMENT METHODOLOGY

Assessment methodology:

Evaluation of attendance, involvement, and academic progress in: 1) theoric activities, 2) laboratory practice, 3) other activities

6. BOOKLIST
MAIN BOOKLIST:
  • Current Protocols in Protein Science. Edition: 1. Author: Coligan JE, Dunn BM, Speicher DW, Wingfield PT. Publisher: John Wiley and sons, Inc.  (Library)
  • Proteomics in Practice. Edition: -. Author: Westmermeier R, Naven T, Höpker H-R. Publisher: Wiley-VCH Verlag GmbH & Co. KGaA.  (Library)
  • Principles and techniques of biochemistry and molecular biology. Edition: 6th ed. Author: -. Publisher: Cambridge: Cambridge University Press, 2005  (Library)
ADDITIONAL BOOKLIST:
  • Introduction to protein structure. Edition: 2ª ed. Author: Branden, Carl. Publisher: New York ; London: Garland Publishing, cop. 1999  (Library)