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Syllabus 2015-16 - 70122004 - Detection and Quantification of Microorganisms in Food: New Technologies (Detección y cuantificación de microorganismos en alimentos: nuevas tecnologías)
- Level 1: Tutorial support sessions, materials and exams in this language
- Level 2: Tutorial support sessions, materials, exams and seminars in this language
- Level 3: Tutorial support sessions, materials, exams, seminars and regular lectures in this language
| DEGREE: | Máster en avances en seguridad de los alimentos |
| FACULTY: | Centro de Estudios de Postgrado |
| ACADEMIC YEAR: | 2015-16 |
| COURSE: | Detection and Quantification of Microorganisms in Food: New Technologies |
| NAME: Detection and Quantification of Microorganisms in Food: New Technologies | |||||
| CODE: 70122004 | ACADEMIC YEAR: 2015-16 | ||||
| LANGUAGE: English | LEVEL: 2 | ||||
| ECTS CREDITS: 5.0 | YEAR: 1 | SEMESTER: AN | |||
| NAME: BENOMAR EL BAKALI, NABIL | ||
| DEPARTMENT: U130 - CIENCIAS DE LA SALUD | ||
| FIELD OF STUDY: 630 - MICROBIOLOGÍA | ||
| OFFICE NO.: B3 - 256 | E-MAIL: nben@ujaen.es | P: 953212007 |
| WEBSITE: - | ||
| ORCID: https://orcid.org/0000-0002-7297-6171 | ||
| LANGUAGE: English | LEVEL: 2 | |
1.- DNA extraction
- DNA extraction from bacteria (phenol-chloroform)
- DNA extraction from bacterial colony
- DNA extraction from food
- Determination of DNA concentration
- DNA gel electophoresis
2.- Molecular identification of bacteria
- Bacterial identification by PCR and DNA Sequencing of 16S rRNA gene
3.-Genomic fingerprinting of bacteria
- RAPD-PCR fingerprinting(Randomly Amplified Polymorphic DNA)
4.- Culture independent study of microbial populations
- DNA isolation from food
- PCR amplification of the microbial community
- TTGE analysis (Temporal Temperature Gradient Gel Electrophoresis)
5.- In situ hybridization
|
ACTIVITIES
|
HOURS ATTENDANCE
|
HOMEWORK |
TOTAL HOURS |
ECTS CREDITS
|
COMPETENCIES (CODE)
|
|
Activity 1: Lecture (Theory) |
10
|
15 |
25 |
1 |
GE1, GE2, E4, E5, TR1, TR2, TR3, TR5 Y TR6 |
|
Activity 2: Lecture (Practice)
|
40 |
40 |
80 |
3.2 |
GE1, GE2, E4, E5, TR1, TR2, TR3, TR5 Y TR6 |
|
Activity 3: exercises at home
|
|
15 |
15 |
0.6 |
GE1, GE2, E4, E5, TR1, TR2, TR3, TR5 Y TR6 |
|
Activity 4: Exam |
5 |
|
5 |
0.2 |
GE1, GE2, E4, E5, TR1, TR2, TR3, TR5 Y TR6 |
|
Total |
55 |
70 |
125 |
5 |
GE1, GE2, E4, E5, TR1, TR2, TR3, TR5 Y TR6 |
Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support
|
|
CRITERIA |
INSTRUMENT |
PERCENTAGE |
|
Lecture (Practice)
|
Laboratory assistance, active participation in the laboratory and delivery of questionnaires. Competencies evaluated: GE1, GE2, E4, E5, TR1, TR2, TR3, TR5 Y TR6 |
Practice exam
|
90% |
|
Exercises at home |
Delivery of the exercises made
Competencies evaluated: GE1, GE2, E4, E5, TR1, TR2, TR3, TR5 Y TR6 |
Review and correction of exercises delivered |
10% |
- Molecular Techniques in the Microbial Ecology of Fermented Foods [Recurso electrónico] . Edition: -. Author: Cocolin, Luca. Publisher: New York, NY : Springer Science+Business Media, LLC, 2008. (Library)
- Brock Biology of Microorganisms. Edition: 12th ed.. Author: -. Publisher: San Francisco, CA : Pearson Benjamin Cummings, c2009. (Library)
- Molecular methods for microbial identification and typing. Edition: -. Author: Towner, K. J.. Publisher: London [etc.]: Chapman & Hall, 1993 (Library)
- Molecular Identification, Systematics, and Population Structure of Prokaryotes [Recurso electrónico]. Edition: -. Author: Stackebrandt, Erko. Publisher: Berlin, Heidelberg : Springer-Verlag Berlin Heidelberg, 2006. (Library)
- New high throughput technologies for DNA sequencing and genomics [Recurso electrónico]. Edition: 1st ed.. Author: -. Publisher: Amsterdam ; Boston : Elsevier, 2007. (Library)
- Real-Time PCR : advanced Technologies and Applications. Edition: -. Author: -. Publisher: WYMONDHAM: CAISTER ACADEMIC PRESS, 2013 (Library)