Universidad de Jaén

Therorical Contents

Item 1. Biological Basis of Human Reproduction. Endocrine system. Male reproductive system. Female reproductive system.

Item 2.Gametogénesis. Spermatogenesis. Ovogénesis.Ovulation. Endocrine control of ovulation

Item 3. Fertilization. Embryonic Development. Transportation sperm and egg. Sperm capacitation and activation. Stages of embryonic development. Gastrulation. Neurulation. Organogenesis

Item 4.Bases Sterility. Identification of the causes of infertility. Female infertility. Male infertility. Specific categories of infertility: genetic infertility. Diagnosis sterility. Infertility Treatment: ovulation induction, artificial insemination fertilization "in vitro" sperm injection. Transfer

Item 5. Seminal analysis and testing of semen. Sperm Bank

Item 6.Techniques Assisted Reproduction: MACS. IMSI. DGP. "Screening" aneuploidy. "Hatching" assisted

Item 7. In Vitro Embryo. Observation and selection of embryos. Embryo culture. Co-culture techniques. Freezing and storing embryos. Defreeze embryos

Item 8. Multiplicación Gamete and Embryo. Reproductive process. Handling. Cloning. Nuclear transfer. Cloning techniques. Cloning applications. Ethical considerations of cloning

Item 9. Sex Determination. Gonadal properties. Phenotypic characteristics. Molecular sex determination. Determination sexing techniques

Item 10.Cryobiology. Paternal and maternal preservation inheritance-Preservation embryos. Embryo Microscopic evaluation Vitrification

Item 11. Genetic diagnosis . Pre-implantation diagnosis. Prenatal diagnosis methods.

Item 12. Laboratory Biosafety in Assisted Reproduction. General principles of biological safety. Biosafety in cryopreservation. Transport of reproductive biological material. Protocols. Evaluation of biological risks

Item 13. Legal and Ethical Aspects of Assisted Human Reproduction. Spanish and European legislation. Ethical issues in research and experimentation

Item 14. Basic and applied investigation in Human Reproduction

PRACTICE CONTENTS

Practice 1. Performing Seminogram. Comparison of fresh semen and cryoperserved samples with different composition diluents

Practice 2. Study changes in sperm motility and morphology at different pHs. In vitro study

Practice 3. Effect of increased scrotal temperature on sperm

Practice 4. Determinate the expression of androgen receptors in male reproductive system by immunohistochemistry

Practice 5. Determinate Estrogen Receptor by Immunohistochemistry in female reproductive system

Practice 6. Astral cycle of the mouse female reproductive system

Practice 7. Histological Study of Female reproductive system

Practice 8. Quantification the expression of estrogen in the ovary, uterus and fallopian and androgen receptors in testis

Practice 9 and 10. Visit the assisted reproduction unit

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

The students must demonstrate knowledge of the theorical content by exam (60% of the final grade). For the evaluation of the knowledge of the subject will be a theoretical written exam. The student must achieve a grade equal to or higher than 5 out of 10 to pass the theoretical part.

 Is requisite the Obligatory Assistance to laboratory practices. The student must work and present a comprehensive notebook practices where answers to the questions that arise and present the results in practice sessions conducted which will be evaluated and will match 20% of the final grade.

Those students who do not make the practices will not have the 20% for practices in the computation of the final grade.

 The seminar activities is 10% of the final grade will be evaluated by the teacher which will take into account attendance, performing the same, and the exhibition of the works. Represents 10% of the final grade. Students who do not perform these activities on a continuous basis may undertake an oral examination consisting of a presentation of a topic related to the theoretical and practical content of the course.

Attendance at practices and  seminars activities will be evaluated and 10% of the final grade

It is possible to pass the course with continuous assessment, without requiring the final theory exam as long as the performance of all academic activities: theoretical, practical and seminars and are overcome with a grade equal to or greater than 5 out of 10