Universidad de Jaén

THEORETICAL PROGRAM

UNIT I: INTRODUCTION TO THE CLINICAL AND ENVIRONMENTAL ANALYTICAL CHEMISTRY. ANALYSIS TECHNIQUES

Item 1. BASIC PRINCIPLES OF CLINICAL AND ENVIRONMENTAL ANALYSIS LABORATORY. Units of measurement. Mass measurements. Lab Supplies. Laboratory water. Safety in the clinical laboratory.

Item 2. ANALYTICAL TECHNIQUES (I). Molecular absorption spectrometry. Fluorimetry. Turbidimetry and nephelometry. Luminometry. Atomic spectrometry. Osmometry. Chromatography. Electrophoresis. Electrochemical techniques.

tem 3. ANALYTICAL TECHNIQUES (II). Immunochemical techniques. Agglutination reactions and immunoprecipitation. Immunoassay with labeled reagents. Automatization. 
 
UNIT II: CLINICAL ANALYTICAL CHEMISTRY

Item 4. BLOOD TEST. Sample types. Collection containers. Variables collection. Laboratory criteria for unacceptable specimens. Sample processing and treating. Errors in the clinical laboratory. Quality control program.

Item 5. URINE ANALYSIS. Renal physiology. Urinalysis. Physical examination of the urine. Chemical examination. Microscopic examination.

Item 6. METHODS OF ANALYSIS OF AMINOACIDS, PROTEINS, CARBOHYDRATES, LIPIDS, VITAMINS AND ENZYMES. Definition and classification. Chemical, physical and biological properties. Specific analysis of clinical parameters: total serum proteins, Urea, Albumin, Glucose, Insulin, Phospholipids, Cholesterol, Triglycerides and Vitamins (water-soluble and fat-soluble).

UNIT III: ENVIRONMENTAL ANALYTICAL CHEMISTRY

Item 7. ENVIRONMENTAL ANALYTICAL CHEMISTRY AND ITS SCOPE. The environment. Analytical and Environmental Chemistry. Concept of contamination. Classification of chemical pollutants. Contaminant transport. Criteria for selecting the analytical method.

Item 8. ATMOSPHERIC ANALYSIS: GASES AND PARTICLES. Atmospheric composition, common air pollutants and their sources. Sampling and preconcentration. Gas analysis. Sources of suspended solids. Measurements of total suspended matter. Techniques of particle size discrimination. Particle analysis. Remote sensing techniques.

Item 9. WATER ANALYSIS: MAJOR CONSTITUENTS AND TRACE CONTAMINANTS. Water quality: natural and waste waters. Sampling. Analysis of general parameters. Determination of gases and organic compounds nonspecific. Trace metal ions. Organic pollutants at trace levels. Item 10. ANALYSIS OF SOLID SAMPLES. Types of solid samples. Sampling and storage. Determination of organic compounds. Methods of determination.

PRACTICAL PROGRAM

Session 1

Practice 1: Determination of iron in serum.

Practice 2: Determination of albumin in serum.

Session 2

Practice 3: Determination of bicarbonate in serum.

Practice 4: Determination of ascorbic acid in urine by the official method of analysis (AOAC).

Session 3

Practice 5: Enzymatic determination of glucose in blood.

Practice 6: Determination of turbidity in water.

Session 4

Practice 7: Determination of dissolved oxygen in waters.

Practice 8: Determination of total organic matter in a soil sample.

Theoretical Program:

Lectures will be exposed as follows: 1) an initial phase, which addresses the issue from the point of view of the objectives, establishing the framework in which it is situated; 2) a middle phase where we proceed to transmission of knowledge with order and coherence using examples and using relevant teaching resources, and 3) a final phase to complete the development of the lesson and find that we have achieved the objectives and propose, where possible, additional tasks outside of class.



Practical Program:

The student will attend a mandatory practical sessions to be carried out the analysis of chemical compounds in different biological and environmental matrices. It is therefore to acquire an overview of the applicability of the analytical techniques to clinical and environmental field. 
 
 
  Supervised Academically Activities:

Seminars/Presentations: Each student will select a topic of clinical or environmental interest to be finally exposed in class to other colleagues.

Exercises , problems and test.

Students with special educational needs should contact the Student Attention Service (Servicio de Atención y Ayudas al Estudiante) in order to receive the appropriate academic support

The final test will be done on the date set by the Faculty of Experimental Sciences.

The practical sessions will be mandatory. The knowledge gained will be evaluated from lab notebook.

To overcome the Supervised Academically Activities will be taken into account attendance, which is mandatory, as the work to be developed.